结直肠腺癌组织survivin和Ki-67的表达及其与预后的关系  

Expression of survivin and Ki-67 in colorectal adenocarcinoma and its clinical significance

作　　者：薛军[1] 武雪亮[1] 高晓斌[2] 王立坤[3] 屈明[1] 郭飞[1] 张鹏程[1] 杨瑞敏[3] 袁美锦[4] 

机构地区：[1]河北北方学院附属第一医院血管腺体外科,河北张家口075000 [2]河北北方学院附属第一医院肝胆外科,河北张家口075000 [3]河北北方学院附属第一医院超声医学科,河北张家口075000 [4]河北北方学院附属第一医院护理部,河北张家口075000

出　　处：《基础医学与临床》2015年第11期1526-1530,共5页Basic and Clinical Medicine

基　　金：河北北方学院校级重大项目(ZD201310);河北北方学院创新人才项目(CXRC1325);河北省卫计委医学科学研究重点课题计划(20150058);张家口市科技局指令性计划(1311055D)

摘　　要：目的探究存活蛋白(survivin)和Ki-67在结直肠腺癌中的表达及与预后的关系。方法用免疫组化SP法和原位杂交法检测survivin和Ki-67蛋白在30例正常结直肠组织、60例结直肠腺癌中的表达水平,分析survivin和Ki-67与病理参数及结直肠癌预后的关系。结果 60例结直肠癌组织中survivin和Ki-67蛋白阳性表达分别为73.34%(44/60)和80.00%(48/60),显著高于30例癌旁正常组织中的10.00%(3/30)和6.67%(2/30),(P<0.01);survivin mRNA在结直肠癌组织、癌旁正常组织中阳性表达分别为76.67%、6.67%;survivin和Ki-67在结直肠癌组织中的表达呈正相关(P<0.05)。Survivin、Ki-67的表达与结直肠癌的TNM分期、肠壁侵润深度、淋巴结转移、肝转移和腹腔微转移均呈正相关(P<0.05)。survivin和Ki-67阳性表达患者1、3和5年生存率较低。survivin、Ki-67、TNM分期、淋巴结转移、肝转移和腹腔微转移是结肠癌患者预后的独立危险因素。结论 Survivin和Ki-67的异常表达与结直肠腺癌发生、发展及预后密切相关,其有望成为结直肠癌诊疗的新靶点。Objective To investigate the expressions of survivin and Ki-67 in the colorectal adenocarcinoma and its clinical significance. Methods The expressions of survivin and Ki-67 in 60 specimens of coloreetal adenocarcinoma tissues and 30 specimens of normal eolorectal tissues were detected using Immunohistochemical SP technique and in situ hybridization. Results The positive expression rates of survivin and Ki-67 in colorectal adenoeareinoma tissues were 73. 34% and 80. 00%,respectively,while in normal colorectal tissues they were 10. 00% and 6. 67%.The difference was significant（ P〈0. 01）. The positive expression rates of survivin mRNA in colorectal adenoeareinoma and normal tissues were 10. 00% and 6. 67%. Expression of survivin was positively related with expression of Ki-67 in coloreetal adenocarcinoma tissues（ P〈0. 05）. Expression level of survivin and Ki-67 was correlatedwith the TNM staging,liver metastasis,the depth of tumor invasion,lymph node metastasis,and peritoneal micrometastasis（ P〈0. 05）. The patients with positive expression of survivin and Ki-67 had poor progosis with lower one,three,and five year survival rates. survivin,Ki-67,the TNM staging,liver metastasis,lymph node metastasis,and peritoneal micrometastasis were independent prognosis factors for coloreetal adenocarcinoma. Conclusions Thethe high expression of survivin and Ki-67 have a significant correlation with the initiation and progression of colorectal adenocarcinoma,and they may be amolecular-biological indicators in predicting the progress and prognosis of colorectal carcinoma.

关 键 词：结直肠癌 SURVIVIN KI-67 免疫组织化学 预后 

分 类 号：R735.34[医药卫生—肿瘤]