小麦-中间偃麦草抗锈易位系中梁27的鉴定及分析  

Identification and Analysis of Zhongliang 27,a Wheat-Thinopyrum intermedium Translocation Line with Resistance to Stripe Rust

作　　者：李爱博[1] 李金荷 宋建荣[3] 王化俊[2] 张耀辉[3] 尚勋武[2] 曹世勤[4] 李葆春[2] 

机构地区：[1]甘肃农业大学生命科学技术学院/甘肃省作物遗传改良与种质创新实验室/甘肃省干旱生境作物学重点实验室,甘肃兰州730070 [2]甘肃农业大学农学院/甘肃省作物遗传改良与种质创新实验室/甘肃省干旱生境作物学重点实验室,甘肃兰州730070 [3]甘肃省天水市农业科学研究所,甘肃天水741000 [4]甘肃省农业科学院植物保护研究所,甘肃兰州730070

出　　处：《麦类作物学报》2015年第11期1489-1493,共5页Journal of Triticeae Crops

基　　金：甘肃省农牧厅生物技术应用与开发项目(GNSW2009-03);甘肃省自然科学基金项目(0803RJZA036);国家自然科学基金项目(31160362);甘肃省教育厅导师研究计划项目;甘肃省农牧厅生物技术专项(GNSW2011-14)

摘　　要：利用普通小麦与八倍体小偃麦中4杂交,获得小麦品种中梁27。条锈病抗性鉴定结果显示,中梁27具有新的抗病基因。为进一步明确中梁27抗病新基因的来源,采用荧光原位杂交技术对其进行分子细胞学分析。结果表明,中梁27在A基因组有两个易位片段,分别为A/E基因组易位和A/St基因组易位,推测中梁27抗病基因可能来源于这两个易位片段。Wheat variety Zhongliang 27 was bred with common wheat and Octoploid Agroticum Zhong4.By identification of stripe rust resistance,it showed that Zhongliang 27 had novel resistance genes.In order to reveal the origin of new resistance gene further,it was studied by fluorescence in situ hybridization to clear the candidate chromosome segments which the new resistance genes came.The results showed that Zhongliang 27 carried two new translocations A/St and A/E,and it was predicted that the resistance genes was derived from the two translocations.

关 键 词：小麦 八倍体小偃麦 条锈病 易位系 

分 类 号：S512.1[农业科学—作物学] S330