植物活性物质对PM_(2.5)致细胞损伤的抗性作用  

Protective Effects of Phyto-Active Substances Against Cel Damage Caused by PM_(2.5)

作　　者：刘涛[1] 阮研硕[1] 毕婷婷[2] 郭辰[2] 赵晓红[2] 

机构地区：[1]首都师范大学生命科学学院,北京100048 [2]北京联合大学功能食品科学技术研究院,北京100191

出　　处：《环境科学研究》2015年第11期1677-1686,共10页Research of Environmental Sciences

基　　金：北京市教育委员会科技计划重点项目(KZ201211417041);北京联合大学生物活性物质与功能食品北京市重点实验室开放课题(Zk70201501)

摘　　要：为研究植物活性物质对PM2.5致细胞损伤的抗性作用,研究了北京城区PM2.5对CHO(Chinese hamster ovary,中国仓鼠卵巢)细胞的损伤作用,并选择植物活性物质绿原酸、阿魏酸和荭草素探讨其对CHO细胞的保护作用.以CCK-8法确定PM2.5的试验剂量和3种活性物质的最佳保护剂量,然后通过彗星试验、微核试验、流式细胞术及Western Blotting分别检测PM2.5的损伤作用及植物活性物质的保护作用.结果表明:1由CHO细胞存活率可知,PM2.5的有效试验剂量为15μg/m L,绿原酸、阿魏酸和荭草素的最佳保护剂量分别为50、20、10μmol/L.2PM2.5可引起CHO细胞明显的DNA损伤、微核形成增加、细胞周期阻滞所致的细胞增殖指数降低,而经阿魏酸、绿原酸和荭草素预处理后,均可减轻PM2.5对细胞的损伤作用,起到了一定的保护作用.3PM2.5能引起CHO细胞中p53和caspase-3蛋白的表达增加,而阿魏酸、绿原酸和荭草素均可使其表达量有所降低.由PM2.5引起的细胞损伤经绿原酸、阿魏酸、荭草素作用后表达量降低.在3种植物活性物质中,以阿魏酸拮抗PM2.5损伤的效果最佳,绿原酸次之,荭草素相对较差.To study the protective effects of plant active substances on cell damage induced by PM2. 5,PM2. 5samples were collected from downtown Beijing to study its effects on Chinese hamster ovary cells（ CHO）. Chlorogenic acid,ferulic acid and orientin were selected to explore their protective effect on CHO cells. First,the test dose of PM2. 5and the optimum protection doses of three types of phyto-active substances were determined by Cell Counting Kit-8（ CCK-8）. The damage and protective effects of plant active substances were determined with comet assay,micronucleus assay,flow cytometry and Western Blotting. The results showed that PM2. 5at 15 μg/m L resulted in significant DNA damage of CHO cells,micronuclei formation increase and decreased cell proliferation index caused by cell cycle arrest. Chlorogenic acid,ferulic acid and orientin at 50,20 and 10 μmol/L respectively had different levels of protective effects on DNA damage caused by PM2. 5. Nevertheless,PM2. 5induced p53 and caspase-3 expression increased in CHO cells. On the contrary,ferulic acid,chlorogenic acid and orientin reduced these expressions to some extent. This suggests that cell damage caused by PM2. 5was reduced by the pretreatments of 50 μmol/L chlorogenic acid,20 μmol/L ferulic acid and 10 μmol/L orientin. Ferulic acid was the optimum antagonist against damage caused by PM2. 5,followed by chlorogenic acid and orientin. The latter was relatively poor.

关 键 词：PM2.5 DNA损伤 阿魏酸 绿原酸 荭草素 保护作用 

分 类 号：X513[环境科学与工程—环境工程] Q946.83[生物学—植物学]