球形芽孢杆菌BS-10高产培养基的优化  

Optimization of high yield medium for Bacillus sphaericus strain 10

作　　者：刘美[1] 王刘庆[1] 廖美德[1] 陆亮[1] 

机构地区：[1]华南农业大学天然农药与化学生物学教育部重点实验室,广东广州510642

出　　处：《西北农林科技大学学报（自然科学版）》2015年第11期214-220,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：广州市科技局项目(2010J1-E361)

摘　　要：【目的】对杀蚊球形芽孢杆菌BS-10高产培养基进行优化,为该菌株的规模化生产提供理论依据。【方法】以菌株BS-10菌体生物量、干质量和芽孢数为考察指标,对培养基中添加的碳、氮组成成分进行正交试验优化,并对优化所得培养基进行发酵罐扩大培养试验和生物活性测定。【结果】菌株BS-10高产毒力最优培养基(NBFB)配方为胰蛋白胨3g/L,牛肉膏1g/L,鱼粉6g/L,麸皮4g/L;采用NBFB培养基,可将BS-10培养周期缩短至18h,芽孢数达4.12×109 CFU/mg,对致倦库蚊幼虫的毒力较基础培养基增大了100倍。【结论】菌株BS-10在NBFB培养基扩大培养中,不仅获得了高产量,而且缩短了发酵周期,具有较好的应用潜力和经济价值。【Objective】The medium for high mosquito larvicide activity of Bacillus sphaericus strain 10（BS-10）was optimized to guide large-scale production.【Method】In terms of bacterial biomass,dry weight and spore count of strain BS-10,carbon and nitrogen sources of medium were optimized by orthogonal experiment.Then,the biomass,dissolved oxygen,and other related parameters of optimal medium were evaluated through a 7Lfermentor.【Result】The optimal medium（NBFB）of BS-10 consisted of 3g/L tryptone,1g/L beef extract,6g/L fish meal and 4g/L wheat bran.Fermentation period was shortened to 18 h with the optimal medium.The maximum spore count was 4.12×10^9 CFU/mg and the toxicity against Culex quinquefasciatus was increased by 100 times compared to basal medium（NB medium）.【Conclusion】Culturing strain BS-10 in NBFB medium obtained higher larvicide activity and shortened the fermentation period,which has a promising application potential and economic value.

关 键 词：球形芽孢杆菌 菌株BS-10 致倦库蚊 鱼粉 麸皮 

分 类 号：S476.11[农业科学—农业昆虫与害虫防治]