结直肠癌组织SATB2蛋白表达与上皮-间充质转化相关性研究  

Relationship between the expression of SATB2 and epithelial-mesenchymal transition in colorectal cancer tissues

作　　者：王凤[1] 陈世荣[1] 樊峰[1] 沈铭红 吕京澴[1] 

机构地区：[1]南京医科大学附属苏州医院.苏州市立医院本部病理科,江苏苏州215002

出　　处：《中华肿瘤防治杂志》2015年第19期1540-1543,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：苏州市科教兴卫青年科技项目(KJXW2013023)

摘　　要：目的观察富含AT序列的特异性结合蛋白2(special AT-rich sequence-binding protein 2,SATB2)在结直肠癌组织中的表达,分析其与上皮-间充质转化(epithelial-mesenchymal transition,EMT)相关蛋白β-catenin、E-cadherin和Vimentin之间的关系。方法选取2011-01-01—2013-06-30苏州市立医院本部手术切除并经病理确诊的150例结直肠癌石蜡标本,采用免疫组化法检测150例结直肠癌组织中SATB2、β-catenin、E-cadherin及Vimentin蛋白的表达,分析其表达的相关性。结果结直肠癌组织中SATB2、E-cadherin和Vimentin蛋白的阳性表达率分别为61.4%、48.7%和46.0%,β-catenin的异常表达率为48.7%。SATB2阴性表达组、中度表达组和高表达组中β-catenin异常表达率分别为62.1%、42.3%和37.5%,SATB2阴性表达组β-catenin异常表达率显著高于SATB2高表达组和中度表达组,P<0.05。SATB2阴性表达组、中度表达组和高表达组中E-cadherin阳性表达率分别为36.2%、51.9%和62.5%,SATB2高表达与E-cadherin高表达密切相关,rs=0.345,P<0.01。SATB2阴性表达组、中度表达组和高表达组中Vimentin阳性表达率分别为55.2%、48.1%和30.0%,SATB2表达与Vimentin表达呈负相关,rs=-0.388,P<0.01。结论 SATB2表达减少可能增加β-catenin蛋白的异常表达,导致EMT的发生,从而促进结直肠癌的侵袭转移。SATB2有望成为防治结直肠癌浸润和转移的新靶点。OBJECTIVE To investigate the expression of special AT-rich sequence-binding protein 2 （SATB2）in colorectal cancer and analyze the relationship between the expression of SATB2 and epithelial-mesenehymal transition re lated proteins in coloreetal cancer tissues. METHODS The expression of SATB2, t3 catenin, E-eadherin and vimentin in 150 specimens of colorectal cancer were detected by immunohistochemistry. The correlations between them were analyzed by SPSS. RESULTS The positive expression rates of SATB2, E-cadherin, vimentin in samples of coloreetal cancer were 61.4%, 48.7%, 46.0% respectively. The rate of 13-catenin abnormal expression was 48.7%. The aberrant expression lev- els of 13-catenin in tissues with negative, moderate and high expression of SATB2 were 62.1 %, 42.3 % and 37.5 % respec tively. The abnormal expression of β-catenin in cases of SATB2 low expression was significantly higher than that of inter mediate and high SATB2 expression （P〈0.05）. The expression levels of E-cadherin in tissues with negative, moderate and high expression of SATB2 were 36.2% ,51.9% and 62.5% respectively. The high expression of SATB2 was signifi- cantly correlated with high expression of E-cadherin （rs = 0. 345, P〈0.01）. The expression levels of Vimentin in tissues with negative, moderate and high expression of SATB2 were 55.2%, 48.1% and 30.0% respectively. The low expression of SATB2 was significantly correlated with high expression of vimentin （rs =- 0. 388, P〈0. 01）. CONCLUSION SATB2 may inhibit the epithelial-mesenchymal transition by means of reduce the abnormal expression of β-catenin.

关 键 词：结直肠肿瘤 SATB2 上皮-间充质转化 Β-CATENIN 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]