双齿围沙蚕化学成分及其浸膏抗肿瘤活性的研究  

Constituents in Perinereis aibuhitensis and inhibitory activity of all extracts from P.aibuhitensis against tumour cells

作　　者：宋淑梅[1] 马睿霄 金枫清 佟长青[1] 翟兴月[2] 李伟[1] 

机构地区：[1]大连海洋大学食品科学与工程学院,辽宁大连116023 [2]大连医科大学附属第二医院临床营养科,辽宁大连116023

出　　处：《河北渔业》2015年第11期4-7,41,共5页

基　　金：海洋局公益性行业科研专项(项目编号201205022-7)

摘　　要：采用硅胶柱色谱及薄层制备色谱等手段从双齿围沙蚕(Perinereis aibuhitensis)中分离纯化出2种化合物。对所获得的化合物通过理化常数测定、波谱数据分析同文献对照的方法进行了结构鉴定。同时,采用CCK-8方法对双齿围沙蚕浸膏及乙酸乙酯萃取部分分别进行抑制肿瘤细胞活性测试。结果表明,从双齿围沙蚕中分离得到2种化合物分别为:胆甾-4烯3α,6β醇(1)和(E)-17-(4,7-dimethyloct-5-en-2-yl)-10,13-dimethyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol(2),且化合物1是首次从沙蚕中分离得到。抑制肿瘤细胞活性测试表明,70%乙醇浸提获得的双齿围沙蚕浸膏及乙酸乙酯萃取部分对人胃癌细胞AGS、人肝癌细胞HepG2以及人卵巢癌细胞SKOV3均具有抑制细胞增殖作用,且具有剂量和时间依赖性,可作为潜在的抗肿瘤药物进行进一步研究。Two compounds were isolated from Perinereis aibuhitensis by column chromatography on silica gel and thin layer chromatography. Their structures were identified by spectroscopic analysis and physical-chemistry properties. The inhibition on tumour cells of all extracts from P. aibuhitensis were evaluated by CCK-8 assay. The results showed that two compounds were obtained and identified as cholest- 4- ene- 3 α, 6β- diol and （E） - 17 - （4,7 - dimethyloct- 5 - en- 2 yl） - 10,13 - dimethyl- 2,3,4,7,8,9,10,11,12,13,14,15,16,17 - tetradecahydro- 1 H- cyclopenta [a] phenanthren- 3 - ol. All extracts from P. aibuhitensis using 75 % EtOH and ethyl acetate layer exhibited the potent inhibitory activities against HepG2, AGS and SKOV3 cells. The extracts could be considered as potential anticancer candidate for further study.

关 键 词：双齿围沙蚕 化学成分 分离纯化 结构鉴定 抑制肿瘤细胞活性 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]