实验性牙周炎大鼠体内外周血及牙周组织中Th17/Treg的检测  

Detection of Th17/Treg in peripheral blood and gingival tissues of experimental rats with periodontitis

作　　者：王兴羽[1] 赵雅静[1] 张丽萍[1] 高雳[1] 张驰[1] 赵川江[1] 

机构地区：[1]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室,广州510055

出　　处：《中华口腔医学研究杂志（电子版）》2015年第5期11-16,共6页Chinese Journal of Stomatological Research(Electronic Edition)

摘　　要：目的初步探讨实验性牙周炎大鼠外周血及炎症牙周组织中辅助性T细胞17(Th17)和调节性T细胞(Treg)的表达。方法采用丝线结扎并牙龈卟啉单胞菌菌液涂抹法建立SD大鼠牙周炎模型,采集外周血、龈沟液以及颌骨样本。体视显微镜及苏木精-伊红染色法观察牙周组织病理改变,流式细胞术检测外周血Th17细胞与Treg细胞的比例,酶联免疫吸附法检测外周血及龈沟液中白细胞介素17(IL-17)、IL-10、转化生长因子β(TGF-β)表达水平,实时荧光定量聚合酶链反应(PCR)法检测牙周组织中RORγt、Foxp3 m RNA的表达。应用SPSS 22.0软件独立样本t检验及方差分析对数据进行统计学分析。结果实验大鼠牙周组织病理改变符合牙周炎病理改变特征,牙槽骨吸收明显。实验组外周血Th17(F=30.88,P=0.00)、Treg细胞(F=147.03,P=0.00)比例以及Th17/Treg比率较对照组均显著上升(F=219.53,P=0.00);实验组牙周组织中RORγt(F=35.61,P=0.04)和Foxp3m RNA(F=216.60,P=0.01)表达较对照组表达显著上升;RORγt/Foxp3 m RNA比率较对照组升高,但差异无统计学意义(F=0.63,P=0.44);实验组外周血血清中IL-10浓度较对照组显著上升(F=6.41,P=0.02),而IL-17(F=0.08,P=0.78)、TGF-β(F=3.48,P=0.06)浓度与对照组差异无统计学意义;实验组龈沟液中IL-17(F=9.03,P=0.01)较对照组显著上升;IL-10(F=5.85,P=0.08)及TGF-β含量(F=9.28,P=0.06)含量较对照组升高,但差异无统计学意义。结论实验性牙周炎大鼠外周血及牙周炎症组织中Th17与Treg表达均显著上调,可能与牙周炎症组织病理改变以及相关全身系统性疾病存在相关性。Objective To observe the presentation of Th17 and Treg cells in peripheral blood and gingival tissues of SD rats with experimental periodontitis. Methods Experimental periodontitis of SD rats was established by silk thread ligation and P.gingivalis daubing. Th17 and Treg cells in peripheral blood were detected by the flow cytometry. PCR was used to measure the presentation of RORγt、Foxp3 mRNA in the gingival tissues. The concentrations of IL-17,IL-10 and TGF-β in peripheral blood and gingival crevicular fluid were measured using ELISA method. Results Th17（F=30.88,P=0.00）and Treg cells （F=147.03,P=0.00） and the ratio of Th17/Treg （F=219.53,P=0.00） in the group of peripheral blood were increased compared with the control group. Presentation of RORγt（F=35.61,P=0.04）and Foxp3（F=216.60,P=0.01）mRNA in the inflamed gingival tissues were increased compared with the control group,however,no significant difference of the ratio of RORγt/Foxp3 mRNA existed between two groups（F=0.63,P=0.44）. The concentrations of IL-10 in peripheral blood（F=6.41,P=0.02）and IL-17（F=9.03,P=0.01）in gingival crevicular fluid of the experimental group significantly increased,and there were no differences of the levels of IL-17（F=0.08,P=0.78）,TGF-β（F=3.48,P=0.06）in peripheral blood and IL-10（F=5.85,P=0.08）in gingival crevicular fluid between two groups.＆nbsp;Conclusion Expression of Th17 and Treg cells both in peripheral blood and in inflamed gingival tissues of rats with periodontitis were up-regulated,which may be associated with the progression of periodontitis, as well as some related systemic diseases.

关 键 词：辅助性T细胞17 调节性T细胞 牙周炎 白细胞介素17 叉头状转录因子3 

分 类 号：R781.42[医药卫生—口腔医学]