盐度渐变与骤变对脊尾白虾渗透、代谢及免疫相关酶活力的影响  

Effect of salinity changes on osmotic-,metabolic-,and immune-related enzyme activities in Exopalaemon carinicauda

作　　者：李玉全[1] 李永生[1] 赵法箴[2] 

机构地区：[1]青岛农业大学海洋科学与工程学院,青岛266109 [2]中国水产科学研究院黄海水产研究所,农业部海洋渔业可持续发展重点实验室,青岛266071

出　　处：《生态学报》2015年第21期7229-7235,共7页Acta Ecologica Sinica

基　　金：国家自然科学基金项目(31101916);山东省现代农业产业技术体系虾蟹类创新团队(SDAIT-15-011);山东省自然科学基金项目(ZR2010CM060);青岛市科技成果转化引导计划(14-2-4-87-jch)

摘　　要：为探讨盐度变化对脊尾白虾(Exopalaemon carinicauda)渗透、代谢及免疫相关酶活力的影响,实验设置了盐度渐变和骤变两个实验。渐变实验,设置5、10、15、20、25、30、33(CK)、40和45共9个盐度梯度;骤变实验,盐度从33突变至0、5、15、25和45,检测血清ATP酶(包括Na+/K+-ATP酶和总ATP酶)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)及超氧化物歧化酶(SOD)活力。结果表明,渐变情况下,盐度为5时,ATP酶活力出现最高值,然后随着盐度的升高表现出先降低后升高的趋势。总ATP酶活力在盐度为15—30之间较稳定,并在此范围内达到最低值。AKP和ACP活力几乎不受盐度渐变的影响。SOD活力随盐度的升高,先上升后下降,并在盐度为33时达到最大值。骤变情况下,ATP酶活力随时间波动较大,AKP和SOD随时间波动较小,而ACP几乎不受影响。结果说明,盐度骤变对脊尾白虾酶活力的影响较盐度渐变明显,ATPase和SOD活力更易随盐度而变化,代谢酶(AKP、ACP)受盐度变化的影响较小,说明渗透调节和免疫相关酶活力对盐度变化反应敏感,养殖过程中应尽量保持盐度稳定。Exopalaemon carinicauda is an economically important shrimp species that is naturally distributed in the estuaries and coastal areas of China, especially in the Yellow Sea and the Bohai Sea, and contributes to one-third of the gross output of polycuhure ponds in eastern China. Despite its economic importance, basic biological knowledge about this species remains limited. Salinity is one of the most important and changeable water quality factors affecting the physiology of aquatic organisms. Salinity variation may cause a variety of physiological responses, such as plasma enhanced stress-related hormones, stimulation of energy metabolism, and disruption of electrolyte equilibrium. Consequently, marine organisms have developed various survival mechanisms against salinity variation. For example, crustaceans adjust osmolarity and maintain an intra-corporal stable state by varying related enzyme activities. E. carinicauda live in estuaries and coastal areas with highly variable salinity ; yet, little is known about the osmotic adjustment mechanisms of this species. Enzyme activity regulation is one of the most important osmotic adjustment mechanisms. To investigate the effects of acute and gradual salinity changes on enzyme the enzyme activity of E. carinicauda, two different experiments （acute change and gradual Change） were performed. In the gradual change experiment, we used two different treatments. In the first treatment, the salinity was gradually raised from 33 to 40 and 45. In the second treatment, the salinity was gradually reduced from 33 to 5, 10, 15, 20, 25, and 30. For each salinity level, a group of 45 animals were randomly selected and cultured for two weeks. In the acute change experiment, five groups of 45 animals were randomly selected at 33 salinity, which was then abruptly changed to 0, 5, 15, 25, and 45. In each group, blood was collected from the animals for further enzyme activity analysis. ATPase （Na^＋/K^＋-ATPase and total ATPase）, alkaline phosphatase （AKP）, acidic phosphatase

关 键 词：脊尾白虾 盐度 酶活力 

分 类 号：S917.4[农业科学—水产科学]