甘氨酸受体α_1亚基在乳鼠心肌细胞的表达及损害性因素对其表达的影响  

Effects of several harmful factors on expression of glycine receptor α_1 subunit in neonatal rat myocardial cells

作　　者：陈楚天 陆大祥[2] 戚仁斌[2] 王华东[2] 

机构地区：[1]广东省公共卫生研究院 [2]暨南大学医学院病理生理教研室

出　　处：《中国病理生理杂志》2015年第11期2005-2008,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30470718)

摘　　要：目的:研究甘氨酸受体α1亚基(GlyRα1)在乳鼠心肌细胞中的表达以及脂多糖(LPS)、缺氧/复氧(H/R)、异丙肾上腺素(ISO)和高糖(HG)对其表达的影响。方法:体外培养乳鼠心肌细胞,Western blotting方法检测心肌细胞上GlyRα1的表达;心肌细胞分别用LPS、H/R、ISO以及HG处理24 h,采用CCK-8试剂检测细胞活力,Western blotting方法检测心肌细胞上GlyRα1的表达。结果:Western blotting方法检测到乳鼠心肌细胞上GlyRα1的表达;LPS(20 mg/L)、ISO(100μmol/L)以及HG(25mmol/L)处理心肌细胞24 h与心肌细胞H/R 3 h对心肌细胞存活率无明显影响;LPS组、H/R 3 h组以及ISO组心肌细胞上GlyRα1表达均高于对照组(P<0.01),而HG组心肌细胞上GlyRα1表达低于对照组(P<0.01)。结论:乳鼠心肌细胞上存在GlyRα1,并且一定浓度的LPS、ISO与一定时间的H/R均可上调乳鼠心肌细胞GlyRα1的表达,而HG可下调乳鼠心肌细胞GlyRα1的表达。AIM： To study the expression of glycine receptor α1 subunit in neonatal rat myocardial cells and to investigate the effect of lipopolysaccharide （ LPS）, hypoxia/reoxygenation, isoproterenol （ISO） and high concentration of glucose （HG） on the expression of glycine receptor ctl subunit in the neonatal rat myocardial cells. METHODS： Neonatal rat myocardial cells were cultured in vitro. The expression of glycine receptor α1subunit was detected by Western blotting. The neonatal rat myocardial cells were treated with LPS （20 mg/L）, ISO （100 μmol/L） or high concentration of glucose （ 25 mmol/L） for 24 h, or were exposed to hypoxia for 3 h followed by reoxygenation for 3 h. Subsequently, the cell viabil- ity was measured by CCK-8 assay, and the expression of glycine receptor α1 subunit was determined by Western blotting. RESULTS： The expression of glycine receptor α1 subunit in the neonatal rat myocardial cells was positively detectable by Western blotting. Compared with control group, no significant difference of the cell viability （P 〉 0. 05 ） in LPS group, ISO group, hypoxia/reoxygenation group and HG group was observed. The expression of glycine receptor α1 subunit was in- creased （ P 〈 0. 01 ） in LPS group, ISO group and hypoxia/reoxygenatio group, but decreased （ P 〈 0. 01 ） in HG group. CONCLUSION： Glycine receptor α1 subunit exists in the neonatal rat myocardial cells. A certain concentration of LPS or ISO, or hypoxia/reoxygenation for a certain period upregulate the expression of glycine receptor α1 subunit, but HG down- regulates the expression of glycine receptor α1 subunit in cultured neonatal rat myocardial cells.

关 键 词：甘氨酸受体α1 脂多糖 缺氧/复氧 异丙肾上腺素 高糖 

分 类 号：R363[医药卫生—病理学]