微小RNA-21在早期胃癌诊断中的价值评价  

Value of microRNA-21 in diagnose of early gastric cancer

作　　者：包晓玲[1] 樊红[2] 

机构地区：[1]甘肃省肿瘤医院肿瘤内科,兰州730050 [2]兰州大学第二医院消化科,730030

出　　处：《中国医师进修杂志》2015年第11期827-829,共3页Chinese Journal of Postgraduates of Medicine

摘　　要：目的探讨外周血微小RNA-21表达水平在早期胃癌诊断中的价值。方法应用实时定量聚合酶链反应（qRT—PCR）技术对52例原位癌患者及40例健康对照者的外周血微小RNA-21表达水平进行相对定量检测，采用受试者工作特征（ROC）曲线对外周血微小RNA-21表达水平在早期胃癌中的诊断价值进行评价。结果胃癌患者外周血微小RNA-21表达水平明显高于健康对照者（2．2864-0．592比1.3184-0．357），差异有统计学意义（U=136．500，P=0．000）。ROC曲线分析结果显示，外周血微小RNA-21表达水平诊断胃癌的ROC曲线下面积为0．934（95％CI0．887～0．982，P=0．000），最佳诊断界点值为1．912，此时外周血微小RNA-21表达水平诊断胃癌的敏感度为86．5％，特异度为95．0％。结论外周血微小RNA-21表达水平对早期胃癌诊断具有重要的价值。Objective To explore the diagnosis value of expression level of peripheral blood microRNA-21 in early gastric cancer patients. Methods In 52 patients with Tis gastric cancer and 40 healthy subjects, expression levels of microRNA-21 in peripheral blood were quantitatively detected by real-time quantitative PCR（qRT-PCR）,and diagnostic value of expression level of microRNA-21 in peripheral blood were evaluated by receiver operator characteristic （ROC） curve. Results Relative quantity expression level of microRNA-21 in peripheral blood in gastric cancer group and healthy subjects group were 2.286 ± 0.592 and 1.318 ± 0.357, and there was statistical significance （U = 136.500, P= 0.000）. ROC curve analysis showed that ROC curve area of expression level of microRNA-21 in peripheral blood in diagnosing gastric cancer was 0.934（95% CI 0.887 - 0.982, P = 0.000）, and the best diagnostic boundary point value was 1.912. The sensitivity of expression level of microRNA-21 in peripheral blood in diagnosing gastric cancer was 86.5%, and the specificity was 95.0%. Conclusion Expression level of mieroRNA-21 in peripheral blood has important diagnostic value in early gastric cancer.

关 键 词：微小RNA-21 早期胃癌 诊断 受试者工作特性曲线 

分 类 号：R735.2[医药卫生—肿瘤]