HPLC法同时测定脑血栓丸中芍药苷和阿魏酸的含量  

Simultaneous Determination of Paeoniflorin and Ferulic Acid in Naoxueshuan Pill by HPLC

作　　者：刘建萍[1] 陈涛[1] 

机构地区：[1]天津中医药大学第一附属医院,天津300193

出　　处：《中国药房》2015年第33期4714-4716,共3页China Pharmacy

基　　金：天津市科技创新专项资金项目(No.06FZZDSH00408)

摘　　要：目的：建立同时测定脑血栓丸中芍药苷和阿魏酸含量的方法。方法：采用高效液相色谱法。色谱柱为Waters SunfireTM C18,流动相为乙腈-水-冰乙酸（17∶82∶1,V/V/V）,流速为1.0 ml/min,检测波长为230 nm,柱温为35℃,进样量为20μl。结果：芍药苷、阿魏酸检测质量浓度线性范围分别为0.024~0.240、0.009 4~0.141 0 mg/ml（r均为0.999 9）;精密度、稳定性、重复性试验的RSD〈2%;加样回收率分别为95.00%~97.90%、95.29%~98.24%,RSD分别为0.59%、0.54%（n=6）。结论：本方法操作简单、重复性好,可用于脑血栓丸的质量控制。OBJECTIVE：To establish a method for the simultaneous determination of paeoniflorin and ferulic acid in Naoxueshuan pill. METHODS：HPLC was performed on the column of Waters SunfireTMC18 with mobile phase of acetonitrile-water-glacial acetic acid（17∶82∶1,V/V/V）at a flow rate of 1.0 ml/min,detection wavelength was 230 nm,column temperature was 35 ℃,and the volume injection was 20 μl. RESULTS：The linear range was 0.024-0.240 mg/ml for paeoniflorin（r=0.999 9）and 0.009 4-0.141 0mg/ml（r=0.999 9）;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 95.00-97.90%（RSD=0.59%,n=6）and 95.29-98.24%（RSD=0.54%,n=6）,respectively. CONCLUSIONS：The method is simple and reproducible,and can be used for the quality control of Naoxueshuan pill.

关 键 词：脑血栓丸 芍药苷 阿魏酸 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]