HPLC-DAD法测定馒头中喹啉黄、食用绿S和亮绿3种色素含量的研究  

Study on determination of 3 kinds of pigment content of yellow,green S and bright green in steamed bread by HPLC-DAD method

作　　者：杨勇[1,2] 罗奕[3] 吴琳琳[3] 杨娟艳 史蕙[2] 许乾丽[2] 游正琴 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]贵州省食品药品检验所,贵州贵阳550004 [3]贵州医科大学,贵州贵阳550004

出　　处：《粮油食品科技》2015年第6期71-75,共5页Science and Technology of Cereals,Oils and Foods

基　　金：食品药品非法添加化工染料的快速检测及相关数据库的研究(黔科合SY字[2011]3090号);贵州省食品;保健食品和化妆品监测检测平台(黔科平台[2012]4001);国家食品药品监督管理局研究课题

摘　　要：建立了高效液相色谱(HPLC)串联紫外器(DAD)测定馒头中3种色素含量的方法。以无水乙醇—氨水—水(7:2:1)溶液作为提取溶剂,用超声辅助提取样品中的色素,用HPLC-DAD进行检测,检测波长425 nm、624 nm,色谱柱为PAK CAPCELL C_(18)(5μm,4.6×200 mm),流速1.0 mL·min^(-1),柱温35℃,以甲醇-乙酸铵溶液为流动相进行梯度洗脱。结果表明:3种色素分离度较好,具有良好的线性关系(r=0.999 9),重现性、精密度良好(RSD%均小于3%),检出限食用绿S为0.848 4 ng、喹啉黄为6.833 9 ng、亮绿为1.336 0 ng,平均加标回收率为91%~105%。该方法简单、快速、灵敏度高、回收率高、检出限低,适用于馒头中喹啉黄、食用绿S、亮绿含量的测定。The contents of 3 kinds of pigment in steamed bread were determined by high performance liquid chromatography （HPLC） tandem ultraviolet （DAD）. Anhydrous alcohol - ammonia - water （7： 2：1 ） solution was selected as the extraction solvent. The contents of pigments were extracted by ultrasound - assis- ted extraction. Detected by HPLC - DAD, with detection wavelength 425 nm, 624 nm, column chromatog- raphy： PAK capcell C18 （5 μm, 4.6 ×200 mm）, flow rate 1.0 mL rain-1, column temperature 35℃, with methanol - ammonium acetate solution as mobile phase to gradient elution. The result showed that the resolutions of the three kinds of pigment were good with good linear relationship （ r = 0.999 9）, reproducibility and precision （ RSD less than 3% ） ; the detection limit of edible green was 0. 848 4 ng, quino- line yellow 6. 833 9 ng, and the bright green 1. 336 0 ng; the average recovery rate was 91% - 105%. This method is simple, fast and sensitive, with high recovery rate and low detection limit, which is suitable for the determination of the content of yellow, green S and bright green in steamed bread.

关 键 词：高效液相色谱-紫外检测器(HPLC—DAD) 馒头 色素含量测定 

分 类 号：TS213.2[轻工技术与工程—粮食、油脂及植物蛋白工程] R115[轻工技术与工程—食品科学与工程]