9-顺式视黄酸抑制人胆管癌细胞株QBC939Wnt信号通路和细胞迁移  

9-cis retinoic acid inhibits Wnt signaling pathway and cell migration in cholangiocarcinoma QBC939 cells

作　　者：提爱军[1] 向丹[1] 李磊华 李明[1] 邵志鸿[3] 庄少勇 李文岗[2] 

机构地区：[1]厦门大学医学院,361102 [2]福建医科大学福总临床医学院 [3]厦门大学附属成功医院肝胆胰血管外科厦门市胆道疾病重点实验室

出　　处：《中华实验外科杂志》2015年第11期2632-2635,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81272246）;厦门市科技计划资助项目（3502220124049）

摘　　要：目的 探讨9-顺式视黄酸（9-cis RA）抑制人胆管癌细胞株QBC939增殖与迁移的能力及其机制.方法 体外培养QBC939细胞株,采用噻唑蓝（MTT）、TOPflash荧光素酶报告基因法、实时定量聚合酶链反应（Real-time PCR）、细胞划痕、Transwell迁移实验以及Western blot法检测9-cis RA对QBC939存活率、Wnt信号通路和上皮-间充质转化（EMT）相关标志物表达影响,以及抑制Wnt信号通路对QBC939的迁移能力的影响.结果 人胆管癌细胞株QBC939经9-cis RA处理后,其增殖、迁移能力和Wnt信号通路活性减弱,上皮细胞标志物E-cadherin蛋白表达升高;抑制Wnt/β-catenin人胆管癌细胞株QBC939的迁移能力降低.结论 9-cis RA抑制胆管癌细胞增殖和迁移的作用可能与9-cis RA对Wnt/β-catenin信号通路的抑制相关.Objective To investigate the mechanisms underlying 9-cis retinoic acid （9-cis RA） inhibiting cholangiocarcinoma cell QBC939 proliferation and migration.Methods TOPflash luciferase assays, real-time quantitative polymerase chain reaction （Real-time PCR）, Transwell assay, wound strack and Western blotting were used to detect the relative growth rate of QBC 939, the activity of the Wnt-signalling pathway, and the ability of cell migration after Wnt-signalling pathway was inhibited.Results Cholangiocarcinoma cell QBC939 proliferation, migration and the activity of the Wnt-signalling pathway were inhibited after the cells were treated with 9-cis RA.However, the expression of epithelial cell marker was increased.Cell migration was inhibited after the Wnt-signalling pathway was inhibited.Conclusion These results suggest that 9-cis RA inhibited Wnt/β-catenin signal pathway, proliferation and migration in QBC939 cells.

关 键 词：胆管癌 9-顺式视黄酸 WNT信号通路 

分 类 号：R73-37[医药卫生—肿瘤]