血管内皮生长因子C通过调节淋巴内皮细胞促进结肠癌细胞生长的研究  

Vascular endothelial growth factor-C promotes colorectal cancer growth via regulating endothelial lymphatic cells

作　　者：陈满宇[1] 呙帆 黄华俊[3] 

机构地区：[1]襄阳市中心医院湖北文理学院附属医院普外3科,441000 [2]襄阳市中医医院脑病科 [3]襄阳市中心医院湖北文理学院附属医院普外科,441000

出　　处：《中华实验外科杂志》2015年第11期2712-2714,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察淋巴内皮细胞在血管内皮生长因子（VEGF）-C促进肠癌细胞生长过程中是否发挥作用.方法 运用过表达VEGF-C（h-VEGF-C）和抑制VEGF-C（L-VEGF-C）表达的慢病毒分别感染CT26细胞株,Western blot验证慢病毒载体的有效性.然后将经过慢病毒处理过两种细胞株和未经处理的CT26细胞株进行裸鼠注射,注射部位为小鼠尾部直肠处,进行小鼠模型构建.动物模型构建成功后进行组织切片免疫荧光染色;染色指标为LYVE-1（标记微淋巴管）和CD31（标记血管密度）.最后亚甲蓝染色观察小鼠肠道肿瘤数目及大小.结果 Western blot结果验证了过表达和抑制VEGF-C表达慢病毒有效性,免疫荧光结果显示过表达VEGF-C的裸鼠淋巴管密度明显高于空白对照组（9.10 ±0.50比5.82±0.81）,差异有统计学意义（P＜0.01）;抑制VEGF-C的裸鼠淋巴管密度明显低于空白对照组（2.62±0.41比5.80±0.81）,差异有统计学意义（P＜0.01）.同样地,过表达VEGF-C的裸鼠血管密度明显高于空白对照组（2.68±0.23比2.01±0.15）,差异有统计学意义（P＜0.05）;抑制VEGF-C的裸鼠血管密度明显低于空白对照组（2.01±0.15比1.05±0.47）,差异有统计学意义（P＜0.05）.同时,亚甲蓝染色结果显示过表达VEGF-C时小鼠肠道肿瘤数目明显多于抑制VEGF-C表达的小鼠（13.4±5.2比4.5±1.8;P＜0.01）;而且两组之间肠道肿瘤大小也有明显差异.结论 VEGF-C可以通过调节淋巴内皮细胞进行促进肠癌细胞的生长.Objective Vascular endothelial growth factor-C （VEGF-C） plays important roles in regulation of proliferation, invasion and metastasis of colorectal cancer.However, the concrete mechanism is poorly unknown.The aim of this study was to find out whether VEGF-C promotes colorectal cancer growth through interacting with endothelial lymphatic cells.Methods First the lentiviruses overexpressing or inhibiting VEGF-C were constructed.Western blotting was performed to prove the effectiveness of the lentiviruses.Then three types of CT26 CRC cells （VEGF-C overexpression;VEGF-C inhibition and blank control） were injected into the distal posterior rectum of BALB/c-nude mice.After constructing the animal models successfully, immunofluorescence method was employed to detect the distribution of LYVE-1 and CD31 in lymphatic endothelial tissues and vascular endothelial cells respectively.In addition, the number and size of tumors were compared between the VEGF-C overexpression mice and VEGF-C inhibition mice.Results Western blotting showed that the lentiviruses were successfully constructed.The VEGF-C over-expression group had a significantly higher density of lymph-vessel than in the blank control group （9.10 ± 0.50 vs.5.82 ± 0.81;P 〈 0.01）.The VEGF-C down-expression group had a significantly lower density of lymph-vessel than in the blank control group （2.62 ± 0.41 vs.5.80 ±0.81;P 〈0.01）.Similarly, the VEGF-C overexpression group had a significantly higher density of blood-vessel than in the blank control group （2.68 ±0.23 vs.2.01 ±0.15;P〈0.05）.The VEGF-C down-expression group had a significantly lower density of lymph-vessel than in the blank control group （2.01 ± 0.15 vs.1.05 ± 0.47;P 〈 0.05）.The number of tumors was more in the VEGF-C overexpression group than in the VEGF-C down-regulation group （13.4 ± 5.2 vs.4.5 ± 1.8;P 〈 0.01）.The tumor size was also significant different.Conclusion VEGF-C might promote colorectal cancer growth through regulating endothelial lymphatic cells.

关 键 词：血管内皮生长因子-C 肠癌 淋巴管 内皮细胞 

分 类 号：R735.2[医药卫生—肿瘤]