CXC趋化因子受体2和白细胞介素-22结合蛋白联合表达及其在胃癌进展中的作用  

The role of co-expression of CXC chemokine receptor 2 and interleukin-22 binding protein in gastric adenocarcinoma progression

作　　者：杨世斌[1] 邓量[1] 肖隆斌[1] 李明哲[1] 韩方海[1] 何裕隆[1] 

机构地区：[1]中山大学附属第一医院胃肠外科,广州510700

出　　处：《中华实验外科杂志》2015年第11期2823-2826,共4页Chinese Journal of Experimental Surgery

摘　　要：目的 探讨CXC趋化因子受体2（CXCR2）和白细胞介素-22结合蛋白（IL-22BP）在胃腺癌表达及两者之间的关系在胃腺癌中的作用.方法 通过免疫组织化学和聚合酶链反应（PCR）序列检测112例胃腺癌中CXCR2和IL-22BP蛋白的联合表达,并分析临床意义.结果 与无癌组织比较,胃腺癌中分别为82例的CXCR2及85例IL-22BP蛋白过表达,且均大部分在细胞质中表达.64例CXCR2及60例IL-22BP mRNA水平呈现共表达.两者蛋白联合表达及CXCR2和IL-22BP阳性表达与pTNM分期、淋巴结转移、肿瘤浸润深度明显相关,差异有统计学意义（P＜0.05）.相对于阴性表达,Kaplan-Meier法分析认为蛋白表达的患者具有较差的总生存期.结论 CXCR2和IL-22BP参与到更复杂的机制及作用中,在进展期胃腺癌中是预后较差的预测指标.Objective To explore the co-expression of CXC chemokine receptor 2 （CXCR2） and interleukin-22 binding protein （IL-22BP） in gastric adenocarcinoma and the roles in gastric adenocarcinoma （GA） progression.Methods The expression of CXCR2 and IL-22BP was detected by immuohistochemistry and real-time reverse transcriptase-polymerase chain reaction （RT-qPCR） in 112 cases of GA tissues, and the clinical significance was analyzed.Results As compared with non-cancerous tissue, in GA, 82 cases of CXCR2 and 85 cases of IL-22BP proteins were over expression, and were observed in cytoplasm mainly.64 cases of CXCR2 and 60 cases of IL-22BP proteins mRNA levels was analyzed, and co-expression relation was identified.It is significantly higher expression and more positive cases of CXCR2 and IL-22BP proteins were observed with advanced pTNM stage in GA, as well as lymph node metastasis present to absent and deep muscular invasion to superficial （P 〈 0.05）.In a Kaplan-Meier analysis, compared to negative expression, both proteins identified a group of patients with the shortest overall survival （OS）.Conclusion CXCR2 and IL-22BP may take part in more complex mechanisms and roles, and indicate poor outcome in GA progression.

关 键 词：CXC趋化因子受体2 白细胞介素-22结合蛋白 胃癌 

分 类 号：R734.2[医药卫生—肿瘤]