microRNA-122在脂肪肝患者血清中的变化  

The changes of microRNA-122 in serum of patients with fatty liver

作　　者：许文龙 李祥云[3] 

机构地区：[1]上海华山医院宝山分院 [2]宝山仁和医院检验科,上海200431 [3]杭州师范大学附属医院检验科,浙江杭州310015

出　　处：《中国卫生检验杂志》2015年第21期3653-3655,3658,共4页Chinese Journal of Health Laboratory Technology

基　　金：杭州市科技发展计划项目(20100633B17)

摘　　要：目的探讨血清中microRNA-122成为脂肪肝肝脏损伤标志物的可能性和意义。方法收集60例中度至重度脂肪肝患者,分别在用药后0周、1周、2周、4周和8周5个时间点采集血液,运用实时荧光定量PCR法和酶速率法对microRNA-122、谷丙酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)和γ-谷氨酰转移酶(GGT)进行测定,对结果进行比较和线性相关分析。结果血清中microRNA-122在脂肪肝患者用药后5个时间点(0周、1周、2周、4周和8周)的表达量显著高于对照组,差异有统计学意义(P<0.05);脂肪肝用药后各时间点microRNA-122与ALT、AST、ALP和GGT均为正相关,相关系数(r)分别为0.63、0.67、0.54和0.58。结论 microRNA-122表达升高可以反映脂肪肝患者肝脏损伤,可能成为脂肪肝肝脏损伤早期诊断和治疗评估的一种新的检测标志物。Objective To explore the possibility and meaning of serum microRNA- 122 serving as a potential biomarker of liver injury for fatty liver. Methods Blood was collected from 60 patients with moderate to severe fatty liver in 0 w,1 w,2 w,4 w and 8 w after drug treatment. microRNA- 122,ALT,AST,ALP and GGT levels were mainly detected with real time fluorescence quantitative PCR and enzyme rate method in 60 moderate to severe fatty liver patients at five different time points after treatment. The results were compared and analyzed with statistical methods and linear correlation analysis. Results Serum microRNA- 122 level was obviously higher in 60 fatty liver patients than that in the healthy controls in the 5 time spots,and the differences were statistically significant（ P〈 0. 05）. Meanwhile,in every time spot,the microRNA- 122 levels were positively correlated with ALT,AST,ALP and GGT levels,and the correlation coefficient（ r） were respectively 0. 63,0. 67,0. 54 and0. 58. Conclusion The elevated expression of serum microRNA- 122 could reflect liver injury of fatty liver. Maybe it was a new biomarker in the early diagnosis and therapeutic evaluation of liver injury for fatty liver.

关 键 词：microRNA-122 谷丙酸转氨酶 天冬氨酸转氨酶 碱性磷酸酶 Γ-谷氨酰转移酶 

分 类 号：R575.5[医药卫生—消化系统]