产耐热植酸酶重组毕赤酵母发酵条件优化的研究  

Research on the Optimal Fermentation Conditions of Recombinant Pichia Pastoris Producing Heat- resistant Phytase

作　　者：王兴吉 盛花开 张杰 

机构地区：[1]山东隆科特酶制剂有限公司,山东临沂276400

出　　处：《中国饲料添加剂》2015年第11期16-18,共3页China Feed Additive

摘　　要：本文对毕赤酵母H工程菌30L发酵产植酸酶的发酵条件及该酶的热稳定性进行了研究。研究表明，在菌体湿重达到90g／L时进入补糖阶段，达到220g／L时补糖结束进入混饲阶段，有利于菌体的生长及产酶，诱导时最佳pH为4．7，植酸酶发酵活性达到25000U／g。对所产植酸酶进行热稳定性研究发现，80℃保温30min残留酶活在75％，在50％保温30min残留酶活在95％，具有良好的热稳定性。In this paper, the optimal fermentation conditions of recombinant pichia pastoris H producing heat - resistant phytase in 30L fermentation tank and thermal stability of the enzyme were studied. The results show that it＇s carbohydrate supplement stages when the strain wet weight is 90 g/L , and when the wet weight of strain is 220g/L, its carbohydrate supplement end into the mixed feeding stage. It is good for the strain growing and enzyme producing, the optimum pH for introduction is 4. 7, and the fermentation activity is 25000U/g. Research on heat - resistant phytase shows that phytase has good thermal stability, and its residual enzyme activity still has 75% at 80℃ （30 mins ） ,and 95% at 50℃ （30 rains ）.

关 键 词：毕赤酵母 植酸酶 发酵优化 热稳定性 

分 类 号：Q946.5[生物学—植物学]