不同缺血预调适方案对大鼠肢体骨骼肌缺血再灌注损伤的影响  

EFFECTS OF THREE PROTOCOLS OF ISCHEMIC PRECONDITIONING ON RAT LIMB SKELETAL MUSCLE ISCHEMIA REPERFUSION INJURY

作　　者：劳宏业 阳富春[1] 罗君[1] 阳茂春[1] 刘军廷[1] 胡峰[1] 王静威[1] 赵劲民[1] 

机构地区：[1]广西医科大学第一附属医院创伤骨科手外科,南宁530021

出　　处：《广西医科大学学报》2015年第4期535-539,共5页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81260276)

摘　　要：目的:探讨不同缺血预调适(IP)方案对大鼠肢体骨骼肌缺血再灌注(IR)损伤的影响。方法:将48只健康成年雄性SD大鼠随机分为IR组、IP A组、IP B组、IP C组,每组12只,各组均予股动脉夹闭4h,再复灌最长24h,IP 3组于4h缺血前施以3个不同方案的预处理:IP A组:6个循环2min缺血/2min灌注;IP B组:3个循环5min/缺血5min灌注;IP C组:10个循环1min缺血/1min灌注。各组骨骼肌IR后2h、12h、24h检测血清肌酸激酶(CK)、乳酸脱氢酶(LDH)含量、骨骼肌湿干比(W/D)值、骨骼肌肌肉坏死面积以及骨骼肌组织形态、超微结构变化。结果:IP A组、IP B组、IP C组骨骼肌IR 2h、12h、24h的血清CK及LDH含量上升、骨骼肌W/D值增加、骨骼肌肌肉坏死面积、骨骼肌组织学改变及超微结构变化,均比IR组有明显好转,差异均有统计学意义(P<0.05)。结论:IP方案能减轻大鼠肢体IR损伤,以3个循环5min缺血/5min灌注的IP方案保护作用最佳。Objective：To investigate effect of different protocols of ischemic preconditioning on skeletal muscle ischemia reperfusion injury in a rat model.Methods：Forty-eight healthy adult male SD rats were randomly divided into ischemia reperfusion group（IR）,ischemic preconditioning（IP）of ischemia groups（Group A,B and C）.Each group and subgroups had 12 rats.All rats were subjected to 4-hour ischemia via occlusion of the femoral artery,followed by 24 hours of reperfusion.Additional ischemic preconditioning interventions were applied in IP Group A,B and C,which before the 4-hour ischemia with three different protocols respectively：6cycles of 2min ischemia/2min reperfusion（IP Group A）;3cycles of 5min ischemia/5min reperfusion（IP Group B）;and 10 cycles of 1min ischemia/1min reperfusion（IP Group C）.At2-,12-and 24-hour reperfusion,blood samples were taken for detecting serum CK、LDH,and muscle tissue samples were taken for measuring wet/dry ratio,necrosis area,histology and ultrastucture changes.Results：CK,LDH,W/D ration,necrosis area,histology and ultrastructure changes in IP group A,B and C were significantly better than those in IR group（P〈0.05）.Conclusion：Protocol of IP can confer protection from skeletal muscle ischemia-reperfusion injury in the rat model,and 3cycles of 5min ischemia/5min reperfusion protocol is optimal.

关 键 词：骨骼肌 缺血再灌注损伤 缺血预调适 大鼠模型 

分 类 号：R-332[医药卫生]