阿奇霉素联合左氧氟沙星对大肠杆菌生物膜的干预  

THE INTERVENTION ROLE OF AZITHRONYC IN AND LEVOFLOXACIN ON E.coli BIOFILM

作　　者：邹龙涛[1] 伍巧源[2] 李艳林[3] 廖蕴华[2] 

机构地区：[1]广西医科大学附属肿瘤医院重症学科,南宁530021 [2]广西医科大学第一附属医院肾内科 [3]广西壮族自治区南溪山医院肾内科,桂林541002

出　　处：《广西医科大学学报》2015年第4期550-552,共3页Journal of Guangxi Medical University

基　　金：广西自然科学基金资助项目(No.2013GXNSFAA019169);广西壮族自治区卫生和计划生育自筹科研课题资助项目(No.Z2015582)

摘　　要：目的:研究阿奇霉素(AZM)联合左氧氟沙星(LFX)对腹膜透析(PD)管大肠杆菌(E.coli)生物膜(BF)的影响。方法:(1)二倍稀释法分别测定AZM、LFX对临床分离E.coli最低抑菌浓度(MIC)及最低杀菌浓度(MBC);(2)建模3d后对各组分别进行处理。A组:空白对照组;B组:1/8AZM MIC组;C组:1LFX MBC组;D组:1/8AZM MIC+1LFX MBC组。经药物作用12h,各组分别以连续稀释法行BF内活菌计数,扫描电子显微镜观察PD管表面BF结构。结果:(1)B组与A组比较差异无统计学意义(P>0.05);C组与A组比较活菌数量减少,差异有统计学意义(P<0.01);D组与C组比较活菌数量减少,差异有统计学意义(P<0.05);(2)扫描电子显微镜观察可见D组E.coli BF结构体积较A组减少。结论:AZM可增强LFX对PD管表面E.coli BF内细菌抗菌效应。Objective：To investigate the destructive effects of Azithromycin（AZM）and Levofloxacin（LFX）to E.coli biofilm of the dialysis catheter tube,and its bactericidal effect of BF within E.coli.Methods：（1）Detected the Minimum inhibitory concentration（MIC）and Minimum bactericidal concentration（MBC）of AZM and LFX on E.coli.（2）Divided into four groups—Group A（lank control group）,Group B（1/8MIC AZM group）,Group C（1 MBC LFX group）,Group D（1/8 MIC AZM and 1 MBC LFX group）.After 12-hour medication,SEM observed the surface of the dialysis catheter tube＇s BF,and crystal violet staining BF semi-quantitative,using flat-panel micro-quantitative method to count viable cells on the surface of the carrier.Results：（1）After 12-hour medication,we used flat-panel micro-quantitative method to count cells on the surface and found that there was no statistically significant between Group A and Group B（P〉0.05）;the bacteria reduced in Group C compared with Group A（P〈0.01）;the bacteria were reduced in Group D compared with Group C（P〈0.05）.（2）We used scanning electron microscopy to observe surface E.coli BF structure and size,and found that the bacteria reduced significantly in Group D than in Group A（P〈0.05）.Carrier of silver staining shows cotton-like BF.Conclusion：The killing effect of combination of AZM and LFX was significantly reduced.

关 键 词：大肠杆菌 生物膜 腹膜透析 阿奇霉素 左氧氟沙星 

分 类 号：R-33[医药卫生]