Sirt1和ProEx C在宫颈高度鳞状上皮内病变细胞学诊断中的价值  

Usefulness of Sirt1and ProEx C in the cytologic diagnosis of cervical squamous intraepithelial lesions

作　　者：莫祥兰[1] 韦荣干[1] 卢军营[1] 申小雄 李雪[1] 王琳[1] 周祥祯[1] 

机构地区：[1]广西壮族自治区人民医院病理科,广西南宁530021

出　　处：《中国实验诊断学》2015年第11期1886-1889,共4页Chinese Journal of Laboratory Diagnosis

基　　金：广西科学研究与技术开发计划课题(桂科攻1140003B-68)

摘　　要：目的探讨Sirt1和ProExC在宫颈高度鳞状上皮内病变(HSIL)细胞学诊断中的价值。方法用免疫细胞化学技术检测436例液基细胞涂片(意义未明非典型鳞状上皮[ASCUS]213例,低级别鳞状上皮内病变[LSIL]101例,高级别鳞状上皮内病变[HSIL]122例)Sirt1和ProEx C表达情况;统计其在诊断宫颈HSIL的敏感度、特异度、准确度等评价指标。结果 (1)Sirt1和ProEx C在预测ASCUS及LSIL中HSIL的敏感度分别为81.48%和74.07%,特异度分别为59.58%和71.43%,准确度分别为61.46%和71.65%,平行试验显示:Sirt1和ProEx C联合应用在预测ASCUS及LSIL中HSIL的敏感度为96.30%,特异度为42.51%。(2)Sirt1和ProEx C在诊断宫颈HSIL的敏感度分别为98.97%和100%,特异度为64%和76%,准确度为91.80%和95.08%,平行试验显示:两者联合应用其敏感度为100%,特异度为48.00%。结论 Sirt1和ProEx C联合检测可提高宫颈HSIL细胞学诊断敏感度,降低漏诊率。Objective To study the Usefulness of Sirtl and ProEx C in the cytologic diagnosis of cervical squamous intraepithelial lesions（SIL）. Methods The expression of Sirtl and ProEx C in 436 liquid-based cytologic samples（213 were ASCUS（atypical squamous cells of undetermined significance ）, 101 were LSIL（low-grade squamous intraepithelial lesion）, 122 were HSIL（high-grade squamous intraepithetial lesion））were done by immunocytochemistry, and their sensitivity,specificity and accuracy in the cytologic diagnosis of cervical HSIL were evaluated. Results （1）The sensitivity,specificity, accuracy of Sirtl and ProEx C in predicting the HSIL in ASCUS and LSIL cytologic results were 81.48% and 74. 07% ,59. 58% and 71. 43% ,61. 46% and 71.65% ,respectively;combined with Sirtl and ProEx C in predicting the HSIL in ASCUS and LSIL cytologic results, the sensitivity was 96. 30% and the specificity was 42.51%. （2）The sensitivity, specificity, accuracy of Sirtl and ProEx C in diagnosis of HSIL were 98.97 % and 100 %, 64% and 76%,91.80 %and 95.08 %, respectively; combined with Sirtl and ProEx C, the sensitivity was 100 % and the specificity was 48.00%. Conclusion Sirtl and ProEx C can improve the sensitivity in the cytologic diagnosis of HSIL.

关 键 词：宫颈鳞状上皮内病变 液基细胞学诊断 细胞免疫化学技术 SIRT1 ProEx C 

分 类 号：R737.33[医药卫生—肿瘤]