非小细胞肺癌活检标本中CK7、TTF-1、NapsinA、CK5/6及p63的表达及其意义  

Expression and significance of CK7,TTF-1,Napsin A,CK5/6 and p63 in biopsy specimen of nonsmall cell lung cancer

作　　者：徐晓艳[1] 杜华[1] 宝鲁日[1] 李时荣[1] 李秀霞[1] 师永红[2] 

机构地区：[1]内蒙古医科大学病理学教研室,呼和浩特010059 [2]内蒙古医科大学第一附属医院病理科,呼和浩特010059

出　　处：《诊断病理学杂志》2015年第11期688-691,共4页Chinese Journal of Diagnostic Pathology

基　　金：国家自然科学基金(81201854)

摘　　要：目的检测CK7、TTF-1、Napsin A、CK5/6及p63在非小细胞肺癌(NSCLC)活检标本中的表达及其意义。方法采用免疫组化SP法检测100例NSCLC活检标本中CK7、TTF-1、Napsin A、CK5/6及p63的表达,并结合NSCLC的临床病理特征进行分析。结果 CK7、TTF-1和Napsin A在68例肺腺癌中的阳性率分别为98.5%(67/68)、92.6%(63/68)和97.1%(66/68),表达水平明显高于CK5/6(7.3%,5/68)和p63(8.8%,6/68)(P<0.05);而CK5/6和p63在32例肺鳞状细胞癌中的阳性率分别为96.9%(31/32)和93.8%(30/32),表达水平明显高于CK7、TTF-1和Napsin A的阳性率28.1%(9/32)、9.4%(3/32)和3.1%(1/32)(P<0.05),两者差异均具有统计学意义。结论 CK7、TTF-1、Napsin A、CK5/6及p63在对肺活检标本腺癌和鳞状细胞癌的鉴别中具有重要意义,可作为非小细胞肺癌鉴别诊断的特异性免疫组化套餐。Purpose To detect the expression and significance of CK7,TTF-1,Napsin A,CK5 /6 and p63 in biopsy specimen of non-small cell lung cancer（ NSCLC）. Methods The expression of CK7,TTF-1,Napsin A,CK5 /6 and p63 was detected by immunohistochemical SP method in 100 NSCLC of biopsy specimen,and the clinical pathological features of NSCLC were analyzed statistically. Results The positive rates of CK7,TTF-1 and Napsin A were 98. 5%（ 67 /68）,92. 6 %（ 63 /68） and 97. 1%（ 66 /68） in 68 cases of lung adenocarcinoma,respectively,which was higher than CK5 /6 [7. 3%（ 5 /68） and p63 8. 8%（ 6 /68） ]（ P〈0. 05）,respectively. The positive rates of CK5 /6 and p63 were 96.9%（ 31 /32） and 93. 8%（ 30 /32） in 38 cases of lung squamous cell carcinoma,which was higher than CK7,TTF-1 and Napsin A [28. 1%（ 9 /32）,9. 4%（ 3 /32） and 3. 1%（ 1 /32） ]（ P〈0. 05）,respectively. Both showed significant increasing gradient. Conclusion CK7,TTF-1,Napsin A,CK5 /6 and p63 expressions show important significance at the differential diagnosis between lung adenocarcinoma and squamous cell carcinoma in biopsy specimen.

关 键 词：非小细胞肺癌 CK7 TTF-1 NAPSIN A CK5/6 P63 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]