高效液相色谱法同时测定蒙药复方森登-4提取液中没食子酸、原儿茶酸的含量  

Simultaneously Determine the Contents of Gallic Acid and Protocatechuic Acid in Mongolian Medicine Compound of Sendeng-4by HPLC

作　　者：白埔 徐鹏程[1] 董玉[1] 

机构地区：[1]内蒙古医科大学药学院,呼和浩特010059

出　　处：《内蒙古大学学报（自然科学版）》2015年第6期654-658,共5页Journal of Inner Mongolia University：Natural Science Edition

基　　金：国家自然科学基金项目(81160550);内蒙古自然科学基金项目(2013JQ03)

摘　　要：建立高效液相色谱法同时测定蒙药复方森登-4提取液中没食子酸、原儿茶酸含量的方法.色谱系统为:采用Elite-C18(250mm×4.6mm,5μm)色谱柱,以乙腈(A)-0.3%磷酸水溶液(B)为流动相,柱温27℃,检测波长为270nm,流速为0.7mL/min.本方法可使没食子酸、原儿茶酸有效分离,在检测范围内线性良好,精密度和重复性良好,加样平均回收率分别为100.5%(RSD=1.48%),97.0%(RSD=1.61%).经过方法学验证,该方法可同时、简便、快速、准确的测定蒙药复方森登-4提取液中没食子酸、原儿茶酸的含量.A HPLC method is established for simultaneously determining the contents of gallic acid and protocatechuic acid in Mongolian compound medicine of Sendeng-4. The chromatography were carried out in an mobile phase of acetonitrile （A） and 0.3% phosphoric acid aqueous solution at a flow rate of 0. 7mL / min under the column temperature of 27℃ and the detection wavelength of 270nm using a Elite-C18column （250mm× 4.6mm,5μm）. This method allowed the effective sep- aration of gallic acid and protocatechuic acid with a good linearity in the detection range. The accura- cy and repeatability were satisfactory and the average recoveries attained 100. 5% （RSD= 1.46%） and 97.0% （RSD=1. 61%）. The verification results revealed that this method can simultaneously, simply,rapidly,and accurately determine the contents of gallic acid and protocatechuic acid in Sen- deng-4.

关 键 词：HPLC 蒙药复方森登-4 提取液 没食子酸 原儿茶酸 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]