水溶蜂胶对人牙髓成纤维细胞的毒性作用研究  

Evaluation on cytotoxicity of water-solubile propolis to human dental pulp fibroblasts

作　　者：刘颖婷[1] 张玉皓[1] 

机构地区：[1]云南省第一人民医院(昆明理工大学附属昆华医院)牙周种植科,云南昆明650032

出　　处：《中国现代医学杂志》2015年第30期18-22,共5页China Journal of Modern Medicine

基　　金：云南省科技厅应用基础研究项目(No:2011FZ277)

摘　　要：目的探讨水溶性蜂胶对人牙髓成纤维细胞的毒性作用,为蜂胶的临床应用提供理论依据。方法用一种新的四唑类化合物MTS比色法检测人牙髓细胞在不同浓度的蜂胶、氢氧化钙溶液中体外培养48 h后细胞的相对增殖率(RGR),用5级毒性分类法评级。结果 1 g/L及以下浓度的水溶蜂胶组对人牙髓成纤维细胞的毒性级别在2以下。结论 1 g/L及以下浓度的水溶蜂胶液对人牙髓成纤维细胞的毒性作用较小,是治疗牙髓疾病的安全药物。【Objective】 To evaluate the cytotoxicity of water-solubile propolis to human dental pulp fibroblasts and provide information for local application of propolis to dental diseases.【Methods】 Human dental pulp fibroblasts were incubated in propolis and calcium hydroxide solution at different concentrations for48 hours.The relative growth rate（RGR） was examined by a new method of MTS assay and cytotoxicity was classified by the five-class method.【Results】 The cytotoxicity of 1 g/L and lower-concentration water-solubile propolis solution to human dental pulp fibroblasts was below the second class.【Conclusion】 Water-solubile propolis solution at 1 g/L and lower concentration has weak cytotoxicity for human dental pulp fibroblasts and it is safe for local application to dental pulp diseases.

关 键 词：水溶蜂胶 人牙髓成纤维细胞 细胞毒性 

分 类 号：R781.3[医药卫生—口腔医学]