纤维连接蛋白修饰的微沟槽钛片对人牙龈成纤维细胞活性影响的研究  

Effectes of Fibronectin-immobilized Microgroove Titanium Plate on Human Gingival Fibroblasts

作　　者：黄斯佳 许志强[2] 樊牮 郭建斌[3] 周麟[3] 陈江[3] 

机构地区：[1]泉州医学高等专科学校福建泉州362000 [2]莆田学院附属医院口腔科福建莆田351100 [3]福建医科大学附属口腔医院福建福州350004

出　　处：《口腔医学研究》2015年第11期1113-1117,共5页Journal of Oral Science Research

基　　金：福建省自然科学基金项目(编号:2013J05112);福建省临床重点专科资助项目(编号:20121589)

摘　　要：目的:研究微沟槽钛片表面经三氟代乙烷磺酰氯激活并修饰纤维粘结蛋白(fibronectin,FN)后对人牙龈成纤维细胞(HGF)活性的影响。方法:将FN修饰于经过激活的微沟槽钛表面,XPS分析材料表面元素组成;使用cck8法检测HGF接种材料表面6h、12h、1d、3d、5d、7d后的细胞增值率;荧光染色观察接种3d时的细胞数量及形态。I型胶原纤维ELISA实验及Real-time PCR检测接种5d时材料表面对HGF分泌功能的差异。结果:在实验观察的6个时间段实验组材料细胞增值率高于对照组(P<0.05)。3d时HGF在实验组材料表面的伸展率高于空白组(P<0.05)。5d时FN修饰的两组材料上的细胞外I性胶原纤维的数量及mRNA的表达水平较空白组高(P<0.05)。结论:经三氟代乙烷磺酰氯处理修饰FN的微沟槽钛片可促进HGF的增殖、伸展及分泌功能。Objective：To study the effects of fibronectin（FN）-immobilized microgroove titanium plate on human gingival fibroblasts（HGF）.Methods：FN was immobilized to the microgroove of titanium plate after treated with Tresyl chloride.XPS was used to analyze the surface elements.CCK8 was used to evaluate the cell proliferation of HGFs at 6h,12 h,1d,3d,5dand 7dafter treated.The ELISA and real-time PCR were used to detect the secretion and expression of collagen I in HGFs at 5d.Results：All the cell number in the experimental group was higher than control group at the predetermined time（P〈0.05）.The stretch rate of HGFs in the experimental group was higher than control group at 3d（P〈0.05）.The secretion and mRNA level of collagen I in the experimental group were higher than those of control group at 5d（P〈0.05）.Conclusion：The FN-immobilized microgroove titanium plate could promote the proliferation,adhesion and secretion function of HGFs.

关 键 词：微沟槽 三氟代乙烷磺酰氯 纤维连接蛋白 牙龈成纤维细胞 

分 类 号：R78[医药卫生—口腔医学]