BAPTA-AM对TGF-β诱导肝癌细胞HepG2上皮-间叶化转变的影响  

Effect of BAPTA-AM on Transforming growth factor-β induced the epithelium-mesenchymal transition of hepatocellular carcinoma

作　　者：杨媛[1] 李书俊[2] 邓时莉[1] 文国容[1] 金海[1] 庹必光[1] 

机构地区：[1]遵义医学院附属医院消化内科暨贵州省消化疾病研究所,贵州遵义563099 [2]遵义医学院附属医院烧伤整形外科,贵州遵义563099

出　　处：《遵义医学院学报》2015年第5期460-464,共5页Journal of Zunyi Medical University

基　　金：国家自然科学基金资助项目(NO:81160054;NO:81360311);贵州省联合基金资助项目(NO:黔科合LH字[2014]7574)

摘　　要：目的探讨细胞内钙离子络合剂1,2-二(2-氨基苯氧基)乙烷-N,N,N',N'-四乙酸四乙酰氧甲基酯[1,2-bis(2-aminophenoxy)ethane-N,N,N'N'-tetraacetic acid,BAPTA-AM]对转化生长因子(transformation growth factor-β,TGF-β)诱导人肝癌细胞Hep G2上皮-间叶化转变(epithelium-mesenchymal transition,EMT)的影响。方法 TGF-β、TGF-β联合BAPTA-AM分别作用Hep G2细胞24 h后,采用Real time-PCR方法检测上皮细胞钙粘素(E-cadherin)mRNA的表达变化,采用Transwell方法检测Hep G2细胞迁移及侵袭的能力。结果与对照组比较,TGF-β处理Hep G2细胞24 h后,细胞形态变梭,E-cadherin mRNA表达下调(P<0.05),细胞的迁移、侵袭能力均增加(P<0.01);与TGF-β组比较,TGF-β联合BAPTA-AM处理Hep G2细胞24 h后,E-cadherin mRNA表达上调(P<0.05),细胞的迁移、侵袭能力均减弱(P<0.01)。结论 TGF-β能诱导肝癌细胞Hep G2发生EMT,增强Hep G2的迁移、侵袭能力,BAPTA-AM能抑制TGF-β所诱导Hep G2细胞发生EMT,其机制可能与E-cadherin mRNA的表达上调有关。Objective To explore the effects of intracellular calcium ion chelating agent[ 1,2 -bis （2 -amino- phenoxy） ethane - N, N, N＂ N＇- tetraacetic acid ] （ BAPTA - AM ） on transforming growth factor - β （ TGF - β ） induced the epithelium - mesenchymal transition （EMT） of human hepatocellular carcinoma HepG2 cells. Methods The mRNA expression of E - cadherin in HepG2 cells after treated with TGF - β alone and combined with BAPTA - AM for 24 h were analyzed by Real time - PCR, the migration and invasion ability of HepG2 cells were estimated in transwell assays. Results Compared with control group, the morphology of HepG2 cells were changed spindle, and the E - cadherin mRNA level of HepG2 ceils were decreased significantly after TGF - β treatment for 24 h （P 〈 0.05 ）, which were increased after TGF - β combined with BAPTA - AM treatment for 24 h （P 〈 0.05 ） ; however the migration and invasion capability of HepG2 cells were enhanced distinctly （P 〈 0.01 ） after TGF - β treatment for 24 h, which were obviously weakened （ P 〈 0.01 ） after TGF - β combined with BAPTA - AM treatment for 24 h. Conclusion TGF - β could induce the EMT of HepG2 cells and promote the migration and invasion ability of HepG2 cell, which were inhibited by BAPTA - AM, and the mechanism may be related with the upregulation of E - cadherin mRNA expression.

关 键 词：转化生长因子 上皮-间叶化转变 上皮细胞钙粘素 肝癌细胞 

分 类 号：R735.7[医药卫生—肿瘤]