电针对缺血再灌注损伤大鼠缺血周围区皮质小胶质细胞活化的影响  

Effects of Electroacupuncture on Activation of Microglia in Peri-infarct Cortex of Cerebral Ischemia-reperfusion Injury Rats

作　　者：王鲜[1] 黄佳[1] 柳维林[1] 上官豪 郑薏 王露露[1] 林云娇 陶静[1,2] 陈立典[3] 

机构地区：[1]福建中医药大学康复医学院,福建福州市350122 [2]福建省康复技术重点实验室,福建福州市350122 [3]福建中医药大学,福建福州市350122

出　　处：《中国康复理论与实践》2015年第11期1251-1255,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金项目(No.81273835;No.81403462);福建中医药大学重点学科专项基金项目(No.X2014069-学科)

摘　　要：目的探讨电针对缺血再灌注损伤大鼠缺血周围区皮质小胶质细胞活化的影响。方法 36只雄性Sprague-Dawley大鼠随机分为假手术组(n=12)、模型组(n=12)和电针组(n=12)。模型组与电针组均采用改良Longa法制备左侧大脑中动脉闭塞模型,缺血2 h后恢复血供。电针组电针曲池、足三里穴治疗3 d。HE染色观察缺血周围区皮质神经细胞损伤情况;免疫组织化学法观察缺血周围区皮质小胶质细胞活化标记物ED1的表达;Western blotting检测缺血周围区肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β和IL-6的表达。结果与模型组相比,电针组大鼠神经功能缺损症状改善(P<0.05),缺血周围区皮质神经细胞损伤减轻,ED1阳性细胞数减少(P<0.05),TNF-α、IL-1β和IL-6的表达降低(P<0.05)。结论电针对大鼠脑缺血再灌注损伤具有保护作用,可能与抑制缺血周围区皮质小胶质细胞的活化及促炎因子的释放相关。Objective To explore the effect of electroacupuncture on activation of microglia in peri-infarct cortex after cerebral isch-emia-reperfusion in rats. Methods 36 male Sprague-Dawley rats were randomly divided into sham group （n=12）, model group （n=12） and electroacupuncture group （n=12）. The latter two groups were occluded the left middle cerebral arteries with modified Longa＆#39;s method for 2 hours and reperfused. The electroacupuncture group received electroacupuncture at Quchi （LI11） and Zusanli （ST36） acupoints for 3 days. The nerve cell damage in peri-infarct cortex was observed with HE staining, while the expression of ED1 was determined with immunohisto-chemical staining, and the expression of tumor necrosis factor-α（TNF-α）, interleukin-1β（IL-1β） and IL-6 were determined with Western blotting. Results The neurological deficits score improved significantly in the electroacupuncture group （P〈0.05）, with less nerve cell dam-age, less number of ED1 positive microglia （P〈0.05） and less levels of TNF-α, IL-1βand IL-6 （P〈0.05）, compared with the model group. Conclusion The electroacupuncture at Quchi （LI11） and Zusanli （ST36） acupoints can protect brain from ischemia-reperfusion injury, which might be associated with inhibiting the microglial activation and proinflammatory response in peri-infarct cortex.

关 键 词：脑缺血再灌注损伤 电针 小胶质细胞 炎症 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]