表皮生长因子受体通过MAPK／ERK信号通路调节基质金属蛋白酶1表达的研究  

Epidermal growth factor receptor mediates matrix metalloproteinase 1 expression in SiHa cells through MAPK/ERK pathways

作　　者：张宗峰[1] 王红丽[1] 时小丁[1] 马鑫[1] 李佩玲[1] 

机构地区：[1]黑龙江省哈尔滨医科大学附属第二医院妇产科,150086

出　　处：《国际免疫学杂志》2015年第6期517-521,共5页International Journal of Immunology

基　　金：基金项目：黑龙江省教育厅科学技术研究项目（11551163）

摘　　要：目的探讨表皮生长因子受体（EGFR）在宫颈癌SiHa细胞中对基质金属蛋白酶-1（MMP-1）表达的调节作用，明确其相关的信号传导机制。方法利用表皮生长因子（EGF）作为干预因素，信号通路阻断剂分别阻断EGFR、AKT、ERK、P38和JNK的磷酸化，观察EGFR对MMP-1表达的影响及其下游信号通路的变化。结果EGF在mRNA和蛋白水平上诱导MMP-1的表达增加（P均〈0．05）；MAPK和ERK激酶抑制剂在mRNA和蛋白水平上可阻断这种诱导（P均〈0．05）。结论EGFR通过MAPK／ERK信号通路上调MMP-1的表达。Objective To investigate the regulatory effect of epidermal growth factor receptor （EGFR） on the expression of matrix metalloproteinase 1 （ MMP-1 ） in cervical cancer SiI-Ia cell and to elucidate the relat- ed signal transduction mechanisms. Methods SiHa cells were treated with EGF , and the signal transduction pathway blockers of EGFR, PI3-K, MEK, and JNK were used to investigate the effect of EGFR on the expression of MMP-1 and the phosphorylation of signaling pathways. Results EGF induced the expression of MMP-1 at both the mRNA and protein levels （ all P 〈 0. 05）. MMP-1 induction was blocked by MAPK or ERK kinase in- hibitors （all P 〈 0. 05 ）. Conclusion Conclusion EGFR up-regulated the synthesis of MMP-1 through the MAPK/ERK pathway.

关 键 词：基质金属蛋白酶1 表皮生长因子受体 MAPK/ERK 

分 类 号：R737.33[医药卫生—肿瘤]