HPLC法测定小儿腹泻散中鞣质的含量  

Determination of tannin in infantile diarrhea powder by HPLC

作　　者：雷林洁[1] 霍潘峰 刘园园[1] 陈世新[1] 马亮英[1] 

机构地区：[1]新疆医科大学第二附属医院,830028 [2]新疆医科大学药学院,830011

出　　处：《内蒙古中医药》2015年第11期147-147,165,共2页Inner Mongolia Journal of Traditional Chinese Medicine

基　　金：新疆医科大学科研创新基金(XJC201352);乌鲁木齐市水磨沟区科学技术计划项目资助

摘　　要：目的：建立小儿腹泻散中鞣质的含量测定方法。方法：采用高效液相色谱法,Inert Sustain C18柱（250mm×4.6mm,5μm）,检测波长271nm,流速：1.0m L·min-1,柱温40℃,流动相：甲醇-0.1%磷酸溶液（6∶94）。结果：没食子酸在1.55-62μg·m L-1范围内呈良好的线性关系,r=0.9999,平均回收率为99.24%,RSD=0.64%。小儿腹泻散中鞣质的含量为81.2mg·g-1,RSD=0.56%。结论：该方法操作简单、准确,可用于小儿腹泻散中鞣质的含量测定及质量控制。Objective To establish an HPLC method for the determination of tannin in infantile diarrhea powder. Methods the HPLC separation was performed on a Inert Sustain C18 column （250mm×4.6mm,5μm） in gradient elute mode with a mixture consisting of methol and 0.1mL·L^-1 phosphate acid solution at 40℃, the flow rate was 1.0mL·min^-1, the detection wave length was set at 271nm. Results There were established a good linear relation between 1.55-62.0μg·mL^-1 of gallic acid,r=0.9999, the average recovery was 99.24%, RSD was 0.64%. the content of tannin in infantile diarrhea powder was 81.2mg·g^-1,RSD was 0.56%. Conclusions This method is simple and accurate, which can be used to detemina- tion of contents and quality control of the infantile diarrhea powder.

关 键 词：HPLC 小儿腹泻散 鞣质 没食子酸 含量测定 

分 类 号：R286.0[医药卫生—中药学]