木犀草素对大鼠脑缺血后TRAF6表达的影响及其保护作用机制研究  

Neuroprotective effects and underling mechanisms of luteolin in rat ischemic stroke via inhibiting TRAF6

作　　者：乔会敏[1,2] 李震中[1,2] 郭书英[1,2] 杜媛媛[1,2] 陈林玉[1,2] 张祥建[1,2] 

机构地区：[1]河北医科大学第二医院神经内科 [2]河北省神经病学重点实验室,石家庄050000

出　　处：《脑与神经疾病杂志》2015年第6期425-427,共3页Journal of Brain and Nervous Diseases

基　　金：国家自然基金项目(81371287)

摘　　要：目的观察脑缺血后肿瘤坏死因子受体相关因子6(TRAF6)的表达变化,探讨木犀草素的脑保护机制研究。方法用线栓法制作大鼠大脑中动脉(MCAO)闭塞模型。采用Western blot观察TRAF6蛋白变化,比较各组脑梗死体积、患侧脑水肿和神经功能缺损评分。结果脑梗死后24h,TRAF6的蛋白表达明显上调(2.14±0.05);木犀草素可抑制脑梗死后TRAF6的表达(P<0.05);明显改善神经功能缺失、减轻脑水肿及减小脑梗死体积,(P<0.05)。结论木犀草素通过抑制TRAF6的表达起到了脑保护作用。Objective This study is to evaluate the changes of tumour-necrosis factor receptor-associated factor 6 （ TRAF6） after cerebral ischemia and to explore the underlying mechanisms of luteolin.Methods Male Sprague-Dawley rats were subjected to permanent middle cerebral occlusion （ MCAO ） .Luteolin was systemically administered to explore the effect on TRAF6 at 24 h after cerebral ischemia by western blot.The infarct volume, brain water content, and neurological deficits were measured.Results The expression of TRAF6 was up-regulated at 24h after cerebral ischemia （2.14±0.05）.Luteolin down-regulated TRAF6 expression induced by cerebral ischemia （P〈0.05 ） .Meanwhile, luteolin dramatically alleviated neurological deficit, brain water content and infarct volume （ P〈0.05） .Conclusion Luteollin can protect the brain from the damage caused by ischemia, whose neuroprotection effect may be through downregulation of TRAF6.

关 键 词：脑缺血 肿瘤坏死因子受体相关因子6 木犀草素 

分 类 号：R743.32[医药卫生—神经病学与精神病学]