山茱萸美拉德反应产物对CCl_4致小鼠肝损伤的保护作用  

Protective Effect of Maillard Reaction Products of Fructus Corni on CCl4-induced Liver Injury in Mice

作　　者：杨光明[1] 张玉玲[1,2] 戴婷婷[1] 潘扬[1,2] 蔡宝昌[1] 

机构地区：[1]南京中医药大学江苏省中药炮制重点实验室国家中医药管理局中药炮制标准重点研究室国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210023 [2]南京中医药大学药用菌与中药生物技术研究所,江苏南京210023

出　　处：《中药新药与临床药理》2015年第6期786-790,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：江苏省高校自然科学研究重大项目(12KJA360001);江苏高校优势学科建设工程资助项目(PAPD);江苏高校品牌专业建设工程资助项目(PPZY2015A070)

摘　　要：目的研究山茱萸美拉德反应产物(MRPs)对四氯化碳(CCl4)诱导的小鼠肝损伤的保护作用。方法小鼠连续给药7 d后,采用CCl4诱导急性肝损伤,观察小鼠血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的活性变化,测定肝脏指数及肝组织的丙二醛(MDA)含量和谷胱甘肽过氧化物酶(GSH-Px)水平,并采用HE染色对肝组织进行病理学检查,评价山茱萸MRPs对小鼠急性肝损伤的保护作用。结果与模型组比较,各给药组小鼠肝脏指数及血清ALT、AST活性均显著降低(P<0.05,P<0.01);肝组织MDA含量也显著降低(P<0.01),阳性对照组和超滤高剂量组GSH-Px活力水平显著升高(P<0.01,P<0.05)。病理切片显示,MRPs各给药组可以不同程度地改善肝脏组织病变。结论山茱萸MRPs对急性CCl4中毒小鼠受损肝脏有明显的保护作用。Objective To investigate the protective effects of Maillard reaction products （MRPs） of Fructus Corni against acute liver injury induced by carbon tetrachloride（CCl4） in mice. Methods All of the mice except for the model group were given MRPs of Fructus Corni for 7 days, and then were injected with CCl4 to induce acute liver injury. The protective effects were evaluated by observing the influences of MRPs on serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST） activities, liver index, and hepatic malonaldehyde （MDA） and glutathione peroxidase（GSH-Px） contents in mice with acute liver injury, as well as the pathological examination of liver tissues after hematoxylin-eosin（HE） staining. Results Compared to the model group, significant reductions were showed in liver index, and serum ALT and AST activities in mice of each treated group（P〈0.05, P〈0.01）. The MDA content of liver tissue was also decreased significantly（P〈 0.01 ）. The effects of Fructus Corni MRPs groups were in dose-dependent manner. The GSH-Px activity of positive control group and uhrafiltration high-dose Fructus Corni MRPs group was significant higher than the model group（P〈 0.05, P〈 0.01）. No significant difference was found between uhrafiltration high dose group and positive control group. The pathological examination results showed thatMRPs of Fructus Corni could alleviate the pathological changes of liver tissue to various degrees. Conclusion MRPs of Fructus Corni exert significant protective effects on CCl4-induced acute liver damage in mice.

关 键 词：山茱萸 美拉德反应产物 肝损伤 保护作用 

分 类 号：R285.5[医药卫生—中药学]