温室作业人员农药暴露致外周血淋巴细胞基因组不稳定表型  

Phenotypes of Genomic Instability in Peripheral Blood Lymphocytes of Greenhouse Workers with Exposure to Pesticides

作　　者：翟庆峰[1] 赵健[1] 邢杰[1] 邵丽军[1] 邱玉刚[1] 

机构地区：[1]潍坊医学院公共卫生与管理学院,山东261053

出　　处：《环境与职业医学》2015年第11期1051-1053,1057,共4页Journal of Environmental and Occupational Medicine

基　　金：山东省自然科学基金(编号:ZR2013HQ015);潍坊医学院科技创新基金(编号:K11QC1004);潍坊医学院"健康山东"重大社会风险预测与治理协同创新中心重点研究方向(编号:XT1406002)

摘　　要：[目的]分析温室作业农药暴露对菜农外周血淋巴细胞基因组不稳定性的影响及其细胞毒作用。[方法]对安丘市从事温室蔬菜大棚种植者583名及普通农民576名进行问卷调查,根据农药暴露强度,将蔬菜大棚种植组所有调查对象分为低(n=175)、中(n=181)、高(n=212)3个暴露组。利用胞质分裂阻滞法微核细胞组学试验评价温室农药暴露所致菜农基因组不稳定性和细胞毒性。[结果]随着农药暴露水平的增加,微核率、核质桥、核芽发生率、细胞坏死率均有增加趋势(均P<0.01),双核细胞率和核分裂指数具有降低趋势(P<0.01),而凋亡细胞未发现明显的变化趋势。与对照组比较,中、高暴露组上述各指标差异皆具有统计学意义(均P<0.05)。[结论]温室作业人群农药暴露会诱发基因组不稳定表型,并具有较强的细胞毒性。[ Objective ] To study the phenotypes of genomic instability in peripheral blood lymphocytes and the cytotoxicity for greenhouse workers with long-term exposure to pesticides. [ Methods ] Cluster random sampling method was used to select 583 greenhouse workers as pesticide exposure group and 576 common farmers as control group from Anqiu City and to perform a questionnaire survey. According to the level of pesticides exposure, the exposure group was divided into low （n=175）, moderate （n=181）, and high （n=212） exposure groups. Genomic instability and cytotoxicity of pesticides to peripheral blood lymphocytes were detected by cytokinesis-block micronucleus cytome （CBMN-Cyt） assay. [ Results ] With higher pesticides exposure level, micronucleus frequency, nucleoplasmic bridges, nuclear buds, and necrotic cell rate were increased （all P 〈 0.01）; whereas, binucleated cells and nuclear division index were decreased （both P〉0.01）; no significant changes in apoptosis cells were observed. Compared with the control group, all the parameters mentioned above of the moderate or high exposure groups were significantly different （all P 〈 0.05）. [ Conclusion ] Exposure of the greenhouse workers to pesticides could cause genomic instability and strong cytotoxicity.

关 键 词：温室作业 农药暴露 基因组不稳定性 胞质分裂阻滞微核组学试验 细胞毒性 

分 类 号：R114[医药卫生—卫生毒理学]