结直肠癌患者与健康人群粪便菌群及代谢产物的差异性研究  

Differences between colorectal cancer patients and healthy people in fecal microbiota and metabolites

作　　者：王晓学[1] 汪建平[1] 饶本强[2] 邓丽[2] 黄缘[3] 曾广正[2] 陈诚[2] 

机构地区：[1]中山大学附属第六医院,广州510655 [2]南昌大学第三附属医院胃肠研究所 [3]南昌大学第三附属医院消化科

出　　处：《中华胃肠外科杂志》2015年第11期1106-1110,共5页Chinese Journal of Gastrointestinal Surgery

基　　金：国家自然科学基金（81360337）;江西省科技厅对外合作项目（00322291310917031）

摘　　要：目的比较结直肠癌患者与健康人群粪便菌群及代谢产物的差异。方法分别采用焦磷酸测序和气相色谱质谱联用（GC／MS）的代谢指纹技术分析15例结直肠癌患者和12例健康人粪便菌群和代谢产物变化。结果结直肠癌患者与正常人群粪便菌群多样性和均匀度的差异并无统计学意义（P〉0．05），但在菌种／属水平则有18种细菌相对丰度的差异有统计学意义（P〈0．05）；GC／MS技术能清晰区分结直肠癌患者和正常人粪便代谢轮廓变化，结直肠癌患者粪便短链脂肪酸代谢产物醋酸、戊酸、丁酸、异丁酸、异戊酸及9种氨基酸显著高于健康人群（P〈0．05），而3种不饱和脂肪酸、2种甘油及熊去氧胆酸则明显低于正常人（P〈0．05）。相关性分析表明，粪便菌群与代谢谱变化之问有很强的天联。结论结直肠癌患者与正常人粪便代谢产物及某些细菌种类存在明显差异．深入研究有助于了解肠道菌群参与结直肠癌发生的机制和发现结直肠癌新的诊断及治疗靶点。Objective To compare fecal mierobiota and metabolites between colorectal cancer （CRC） patients and healthy population. Methods Feces from fifteen CRC patients and twelve normal people were analyzed by using pyrosequencing and gas chromatography mass spectrometry （GC/MS）. Results There were no significant differences in the overall microbial community structure associated with the disease state, but 18 bacterial genera were underrepresented or overrepresented in the CRC samples. GC-MS profiling revealed higher concentrations for 9 kinds of amino acids and metabolites of short-chain fatty acids, lower concentrations for 3 kinds of unsaturated fatty acids and 2 kinds of glycerin and ursodeoxycholic acid in stool samples from CRC patients. Correlative analysis between the combined datasets revealed some potential relationships between stool metabolites and certain bacterial species. Conclusions There are significant differences in fecal metabolites and the relative abundance of certain types of bacteria between CRC patients and healthy people, which can provide insight into microbial functions occurring in a cancer environment and will help direct future mechanism studies.

关 键 词：结直肠肿瘤 肠道菌群 代谢组学 

分 类 号：R735.34[医药卫生—肿瘤]