凝血酶预处理对大鼠脑出血后内源性神经再生的影响  

Effect of thrombin preconditioning on endogenous neurogenesis after intracerebral hemorrhage in rats

作　　者：关景霞[1] 张少锋[1] 袁振华[1] 黄婷婷[1] 卢祖能[1] 

机构地区：[1]武汉大学人民医院神经内科,430060

出　　处：《卒中与神经疾病》2015年第5期259-262,共4页Stroke and Nervous Diseases

基　　金：国家自然科学基金资助项目(项目批准号为81301010)

摘　　要：目的探讨凝血酶预处理(thrombin preconditioning,TPC)对大鼠脑出血后内源性的神经细胞再生的影响。方法 SD大鼠随机分为对照组、脑出血(Intracerebral hemorrhage,ICH)组和TPC组,每组分为3、7、14、21、28 d亚组。应用胶原酶脑内立体定向注射制作脑出血模型。TPC组首先将1U凝血酶立体定向注入右侧纹状体,1 d后再制作脑出血模型。所有大鼠应用Brd U腹腔注射在体标记再生的脑室下带(subventricular zone,SVZ)细胞,应用免疫组织化学技术检测Brd U阳性细胞。结果脑出血后3 d同侧SVZ和基底节Brd U阳性细胞开始增加,但与对照组比较差异没有显著性(P>0.05),7 d时Brd U阳性细胞数增加明显,与对照组比较有明显差异(P<0.05),14 d达到高峰,然后逐渐下降,28 d时Brd U阳性细胞虽然仍有增加,但与对照组比较差异没有显著性(P>0.05)。TPC组3 d时Brd U阳性细胞数目开始增加,与脑出血组和对照组比较,差异均具有显著性(P<0.01),14 d达高峰,一直持续至21 d,28 d时Brd U阳性细胞虽然略有下降,但与对照组和脑出血组比较,仍有统计学差异(P<0.05)。结论凝血酶预处理能够增强脑出血后内源性神经再生,可能为脑出血后内源性神经再生的研究提供新的思路。Objective To identify the effect of thrombin preconditioning（ TPC） on endogenous neurogenesis after intracerebral hemorrhage（ ICH） in rats. Methods Sprague-Dawley rats were randomly divided into control,ICH and TPC groups. Each group was divided into 3d,7d,14 d,21d,and 28 d subgroups respectively. ICH model was induced by collagenase stereotaxical injection. In TPC group,1U thrombin was stereotaxically injected into basal ganglia area 1 day before ICH model was conducted. SVZ cells were marked by Brd U intraperitoneal injection. Brd U positive cells were detected by immunohistochemical methods. Results Brd U positive cells increased at 3 d in the ipsilateral SVZ and basal ganglia area after ICH,and there was no significant difference compared with control group（ P〉0. 05）. Brd U positive cells were remarkably increased at 7 d with significant difference compared to control group（ P〉0. 05）,peaked at 14 d and then declined. Brd U positive cells were still increased at 28 d after ICH,and there was no significant difference compared with control group（ P〉0. 05）. Brd U positive cells were increased at 3d in the ipsilateral SVZ and basal ganglia area in TPC group with significant difference compared with control and ICH groups（ P〉0. 01）,peaked at 7 d and continued to the 21 d. Brd U positive cells were slightly decreased at 28 d in the ipsilateral SVZ and basal ganglia area in TPC group,but there was still significant difference compared with control and ICH groups（ P〈0. 01）. Conclusions Thrombin preconditioning can enhance endogenous neurogenesis after intracerebral hemorrhage,which may provide new ideas for the study of endogenous neurogenesis after intracerebral hemorrhage.

关 键 词：脑出血 凝血酶预处理 脑室下带 神经再生 

分 类 号：R743.34[医药卫生—神经病学与精神病学]