血糖水平对糖尿病大鼠脑缺血后血管新生的影响机制  

Effect of blood glucose level on angiogenesis of diabetic rats after ischaemia

作　　者：孟珊珊[1] 赵庆杰[2] 刘洪文[3] 张杰[3] 

机构地区：[1]哈尔滨医科大学附属第一医院群力院区,150070 [2]哈尔滨医科大学附属第一医院神经内科 [3]哈尔滨医科大学附属第一医院大桥分院

出　　处：《卒中与神经疾病》2015年第5期263-267,共5页Stroke and Nervous Diseases

基　　金：黑龙江省卫生厅科研课题(项目编号2011-003)

摘　　要：目的探讨血糖水平对糖尿病大鼠脑缺血后血管新生的影响机制。方法 ELISA法测定血浆VEGF和SDF1水平;western blotting检测脑组织中VEGF和SDF1表达水平;检测脑组织SOD、MDA、GSH-Px活性水平;检测脑缺血区血管密度。结果在糖尿病大鼠脑缺血模型中(1)血浆和脑组织中VEGF和SDF1水平均下降,随着血糖水平的增加,VEGF和SDF1水平逐渐下降,二者呈负相关;(2)随血糖水平的升高,SOD、MDA水平相应降低但GSH-Px水平增加;(3)新生血管数量随血糖升高逐渐减少,与血浆和脑组织VEGF及SDF1的表达趋势相似,与MDA水平呈负相关。结论在糖尿病大鼠脑缺血模型中高血糖可抑制血浆和脑组织VEGF和SDF1的水平,增加MDA水平和氧化应激损伤,新生血管数量减少,高血糖可能通过减少VEGF及SDF1的表达而对血管新生起到抑制作用。Objective To explore the mechanism of blood glucose at different levels impact angiogenesis by detected the form of new blood vessels and the change of VEGF、SDF-1 and ROS expression of diabetes rats after ischaemia,Methods VEGF and SDF1 of plasma were detected by ELISA; VEGF and SDF1 expressions were detected by western blotting; the activity of SOD、MDA、GSH-Px were determined and the vascular density in the brain tissue was counted. Results In the ischemic model of diabetes rats： 1. VEGF and SDF1 level decreased in both plasma and brain tissue,with the increase of blood glucose level. Level of VEGF and SDF1 gradually decline,both negatively correlated with blood sugar level; 2. In contrast to the GSH-PX,SOD and MDA decreases with the rise of blood glucose levels; 3. Number of new blood vessels reduced in brain of rats with higher level of blood glucose,the same as the expression of VEGF and SDF1 in plasma and brain tissue,and negatively correlated to the level of MDA. Conclusions In ischemia model of diabetic rat,hyperglycemia can reduce VEGF and SDF1 of plasma and brain tissue,increased MDA levels and oxidative stress injury,decreased the angiogenesis. Hyperglycemia may inhibit angiogenesis by reducing the expression of VEGF and SDF1.

关 键 词：糖尿病 血管新生 VEGF SDF-1 ROS 

分 类 号：R587.1[医药卫生—内分泌] R743[医药卫生—内科学]