缺血后处理对局灶脑缺血再灌注大鼠Toll样受体2表达的影响  

Effects of ischemic postconditioning on TLR2 expression in rats cerebral with ischemia / reperfusion injury

作　　者：邢变枝[1] 陈晖[2] 汪华新[3] 

机构地区：[1]武汉大学人民医院放射科,430060 [2]武汉大学人民医院泌尿外科,430060 [3]武汉大学人民医院麻醉科,430060

出　　处：《卒中与神经疾病》2015年第5期273-276,共4页Stroke and Nervous Diseases

基　　金：国家自然科学青年基金(30901552)

摘　　要：目的探讨缺血后处理对大鼠局灶性脑缺血再灌注时Toll样受体2(TLR2)表达的影响。方法成年雄性SD大鼠90只,分为假手术组、缺血再灌注组、缺血后处理组各30只;用线栓法建立局灶性大脑中动脉闭塞模型(MCAO),随机分为假手术组(sham)、缺血再灌注组(I/R)、缺血后处理组(IPC),分别于再灌注24、48、72 h后留取大脑皮质组织;采用Longa的等级评分法进行神经行为学评分,免疫组化和蛋白质印记(Western blot)法检测TLR2蛋白的表达水平,逆转录-聚合酶链反应(RT-PCR)检测TLR2 mRNA表达水平。结果 (1)缺血后处理组大鼠神经行为学评分明显改善;(2)缺血再灌注组TLR2蛋白在再灌注24、48、72 h表达水平明显升高(P<0.05);缺血后处理组TLR2蛋白表达在各时间点均减少(P<0.05);TLR2 mRNA的表达趋势与蛋白表达基本一致。结论缺血后处理可以降低TLR2表达水平,这可能是其脑保护作用的部分机制之一。Objective To investigate the protective effect of ischemic postconditioning by examining the express of toll-like receptor 2（ TLR2） in cerebral ischemic reperfusion injury（ I / R）. Methods SD rats model of focal ischemic reperfusion was induced by intraluminal middle cerebral artery occlusion（ MCAO） with a nylon monofilament suture. Ischemic animals were randomly assigned to 3 groups： sham group（ n = 30）,I / R group（ n = 30） and IPC group（ n = 30）. Each group was divided into 3 subgroups by the different times after reperfusion（ t = 24 h,t = 48 h,t = 72 h）. In IPC group,animals were further subjected to postconditioning（ 3 cycles of 30 s ischemia and 30 s reperfusion） after MCAO. Neurologic scores and infarct volumes were assessed at 24,48 and 72 hours. TLR2 was examined by immunohistochemistry,Western blot and RT-PCR. Results IPC treatment improved neurologic scores and reduced infarct volumes compared with the I / R group. Compared with the sham group,the expression of TLR2 increased in the rats undergoing I / R procedure. The level of TLR2 significantly reduced in ischemic postconditioning group. Conclusions Ischemic postconditioning can reduce the expression of TLR2 which may be related to neuroprotective effects.

关 键 词：脑缺血 缺血后处理 缺血再灌注损伤 TOLL样受体2 

分 类 号：R743[医药卫生—神经病学与精神病学]