甲磺酸伊马替尼对高氧暴露新生大鼠肺组织PDGF-B的影响  

Influence of imatinib mesylate in hyperoxia-exposed newborn rats by PDGF-B in the lung

作　　者：沙宁[1,2] 田兆方[1] 李玉红[1] 

机构地区：[1]南京医科大学附属淮安第一医院儿科,223300 [2]江苏省淮安市第二人民医院儿科,223002

出　　处：《实用医学杂志》2015年第21期3493-3495,共3页The Journal of Practical Medicine

基　　金：2014江苏省级条件建设与民生科技专项资金项目(编号:BL2014063);淮安市应用研究与科技攻关(社会发展)计划项目(编号:HAS2014010)

摘　　要：目的:探讨血小板源性生长因子B亚型(PDGF-B)在高氧致新生大鼠肺组织损伤过程中的表达及甲磺酸伊马替尼对其影响。方法:24只新生大鼠依据是否高氧暴露及是否干预用药,分为3组:正常对照组(空气环境+腹腔注射生理盐水)、高氧对照组(95%氧暴露7 d+腹腔注射生理盐水)、高氧干预组(95%氧暴露7 d+腹腔注射甲磺酸伊马替尼),10 d时处死全部大鼠,光镜观察并盲法进行肺组织病理学评分;ELISA法检测血液及肺泡灌洗液中PDGF-B水平;Western blot检测肺组织匀浆PDGF-B蛋白;RT-PCR法检测肺组织匀浆PDGF-B m RNA表达。结果:与正常对照组相比,高氧对照组及高氧干预组肺组织损伤评分增加;肺泡灌洗液与肺组织中的PDGF-B蛋白及肺组织PDGF-B m RNA表达均增高,而高氧干预组较高氧对照组数值下降,差异均有统计学意义(P<0.05)。但3组间血清PDGF-B比较差异无统计学意义。结论:甲磺酸伊马替尼可能通过PDGF-PDGFR轴对高氧诱导的新生大鼠肺损伤产生保护作用。Objective To investigate the influence of high oxygen exposure on signaling pathway of platelet derived growth factor B（PDGF-B） of the lung in newborn rats and the mechanisms of protecting lung injury by imatint bmesylate. Methods Twenty-four newborn Sprague-Dawley rats were randomly divided into three groups, as hyperoxia-exposed ＋ imatinib mesylate group （group A）, hyperoxia-exposed group （group B）, and air-exposed group （group C）. The rats from the group A and B were placed in a sealed Plexiglas chamber with a minimal in- and-outflow, providing six to seven exchanges per hour of the chamber volume and maintaining 02 levels above 95%, while rats in the group C only exposed to air simultaneously. Seven days later, rats in the group A were injected intravenously with imatinib mesylate after hyperoxia exposure, but rats in group B and C received subcutaneous injection with NS alone at the same Lime point. Then all the rats were exposed to air, and were sacrificed three days later. The levels of PDGF-B in bronchoalveolar lavage fluid （BALF） and in serum were detected by ELISA, PDGF-B mRNA in tissue homogenates were detected by RT-PCR, also the value of lung damage score were calculated with histology under light microscope. Results There were significant differences among three groups in the fields of lung damage score （P 〈 0.05）, PDGF-B mRNA （P 〈 0.05） in lung tissue homogenates, and the level of PDGF-B （P 〈 0.05） in BALF, all these parameters in group A and group B were higher than that in group C, There were also significant differences between group A and group B in these parameters （P 〈 0.05）. There was also no significant difference in serum among the three groups. Conclusion Imatinih mesylate protects hyperoxia-in- duced lung injury via PDGF-PDGFR.

关 键 词：甲磺酸伊马替尼 高氧 新生大鼠 肺 PDGF-B 

分 类 号：R722.1[医药卫生—儿科]