白介素8基因mRNA在左右半结肠癌组织中的差异表达及其临床意义  

Clinical significance of differential expression of interleukin 8 m RNA in leftsided and right-sided colon cancer

作　　者：许开宇[1] 李雨晨[2] 伍江平[2] 顾晋[3] 

机构地区：[1]首都医科大学附属北京世纪坛医院肿瘤外科,北京市100038 [2]首都医科大学附属北京世纪坛医院肿瘤研究室,北京市100038 [3]北京大学肿瘤医院结直肠外科,北京市100142

出　　处：《世界华人消化杂志》2015年第30期4822-4830,共9页World Chinese Journal of Digestology

摘　　要：目的:基于右半结肠癌(right-sided colon cancer,RSCC)和左半结肠癌(left-sided colon cancer,LSCC)2个亚群,探索白介素8(interleukin 8,IL-8)基因在2组癌组织中的表达及其临床意义.方法:选择接受根治性手术切除的病理Ⅱ和Ⅲ期结肠癌患者80例,以结肠脾曲为界,分成RSCC组40例和LSCC组40例,采集每个患者的癌组织及其自身配对正常结肠黏膜组织共80对.应用实时定量PCR检测(real-time quantitative PCR,q RT-PCR)的方法检测IL-8基因m RNA在2组患者组织中的表达,并分析其表达和临床病理变量的关系及其对各自预后的影响.结果:无论RSCC还是LSCC,IL-8基因m RNA在癌组织中的表达均显著高于自身正常结肠黏膜的表达(P<0.01);然而,IL-8在RSCC中的表达明显高于LSCC,经检验具有显著性.IL-8高表达患者与RSCC(65%vs 35%,P=0.007),较大肿瘤直径(5.2 cm vs 4.1 cm,P=0.014),和肿瘤浸润淋巴细胞(27.5%vs 10%,P=0.045)显著相关;L S C C患者中I L-8高表达者的中位生存时间明显短于低表达者(37.0 mo vs 59.2 mo,P=0.006),而对RSCC患者的单独分析却发现IL-8高表达者的预后好于低表达者(63.8 mo vs 54.5 mo,P=0.151).多因素分析发现,T4结肠壁浸润(RR=6.514,95%CI:1.209-35.102,P=0.029)和脉管癌栓(RR=6.272,95%CI:1.352-29.092,P=0.019)是RSCC的独立预后因子;IL-8高表达(RR=3.279,95%CI:0.973-11.051,P=0.045)、T4结肠壁浸润(RR=4.546,95%CI:1.335-15.481,P=0.015)、淋巴结转移(RR=5.918,95%CI:1.439-24.334,P=0.014)和脉管癌栓(RR=3.663,95%CI:1.089-12.320,P=0.036)是LSCC的独立预后因子.结论:IL-8基因在RSCC的表达明显高于L S C C,且对两者具有不同的预后意义,是L S C C不良的独立预后因子.可见根据肿瘤部位的不同分析基因表达的临床意义是很有必要.RSCC和LSCC是具有复杂分子差异和不同致癌机制的两个潜在的结肠癌异质性群体.AIM：To investigate the differential expression of interleukin 8（IL-8） between left-sided and right-sided colon cancer and to analyze its clinical significance.METHODS：Eighty patients who received radical resection for stage Ⅱ or Ⅲ colon cancer were involved,including 40 patients with leftsided colon cancer（LSCC） and 40 with rightsided colon cancer（RSCC）.Paired cancer and normal colon mucosal specimens were collected from each patient.The gene expression of IL-8 was detected by real-time quantitative PCR.Subsequently,the relationship between IL-8 expression and clinicopathological variables as well as prognosis was analyzed.RESULTS：The mRNA expression of IL-8 in colon cancer was significantly higher than that in normal colon mucosa（P〈0.01）.IL-8expression was significantly higher in RSCC than in LSCC（P = 0.004）.High IL-8 expression was closely related with RSCC（65%vs 35%,P= 0.007）,bigger tumor size（5.2 cm vs 4.1 cm,P = 0.014） and tumor infiltrating lymphocytes（27.5%vs 10%,P = 0.045）.In patients with LSCC,the survival of patients with high IL-8expression was significantly shorter than that in patients with low IL-8 expression（37.0mo vs 59.2 mo,P = 0.006）.However,among patients with RSCC,patients with high IL-8expression tended to have a better prognosis than patients with low expression（63.8 mo vs 54.5 mo,P = 0.151）.Multivariate analysis demonstrated that T4 infiltration（RR = 6.514,95%CI：1.209-35.102,P = 0.029） and lymphatic/vascular invasion（RR = 6.272,95%CI：1.352-29.092,P = 0.019） were independent prognostic factors of RSCC,and high IL-8expression（RR = 3.279,95%CI：0.973-11.051,P = 0.045）,T4 infiltration（RR = 4.546,95%CI：1.335-15.481,P = 0.015）,lymph node metastasis（RR = 5.918,95%CI：1.439-24.334,P = 0.014）and lymphatic/vascular invasion（RR =3.663,95%CI：1.089-12.320,P = 0.036） were independent prognostic factors of LSCC.CONCLUSION：The gene expression of IL-8is significantly different between RSCC and LSCC.High IL-8 expression h

关 键 词：白介素8 左半结肠癌 右半结肠癌 预后 

分 类 号：R735.35[医药卫生—肿瘤]