7种肉类线粒体DNA的提取及鸭源性成分检测  

Extraction of Mitochondrial DNA in Seven Kinds Meat and Detection of Duck Meat Composition

作　　者：曲莉[1] 李潇涵 王雪松[2] 高丽君[2] 艾金霞[2] 李明成[2] 

机构地区：[1]北华大学公共卫生学院,吉林吉林132013 [2]北华大学医学检验学院,吉林吉林132013

出　　处：《食品研究与开发》2015年第20期107-110,共4页Food Research and Development

基　　金：吉林省科技发展计划项目(20140307008YY)

摘　　要：探讨肉类线粒体DNA(mitochondrial DNA,mt DNA)提取方法,对7种生肉中的鸭肉成分进行鉴别。应用改良盐析法和柱层析法提取肉类mt DNA,紫外分光光度计检测mt DNA的浓度和纯度,设计鸭肉cyt b特异性引物,采用PCR对7种生肉中的鸭肉成分进行鉴别。结果:两种提取肉类mt DNA的方法均能保证DNA的浓度及纯度达到PCR的要求,柱层析法成本略高于改良盐析法;鸭引物可以从7种生肉中鉴别出鸭源性成分,经过多次重复试验证明了鸭肉引物的特异性,为肉制品质量监控的应用提供了实验基础。Two methods were used to extract the mtDNA in meat , the best method was determined through comparing the concentration and purity of mtDNA .The specific primers of duck cyt b was designed to identify the most common adulterated meat on the market , duck compositions were identified in 7 kinds of raw meat by PCR technology. The results show that the two methods of extracting meat mtDNA can achieve the requirement of the PCR in the concentration and purity of DNA. The cost of column chromatography was higher than the improved salting out method. Duck source composition can be identified from 7 kinds of raw meat by duck primer, the specificity of the duck primers was proved through the repeating experiment. This test provides the experimental basis for the application for the meat products quality monitoring.

关 键 词：改良盐析法 柱层析法 线粒体DNA PCR 鸭肉 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]