二氢杨梅素预干预对人肺癌A549细胞放射治疗的敏感性作用  

Radiosensitivity on A549 cells by dihydromyricetin pre-intervention in vitro

作　　者：孙大永[1] 毕红东[2] 梁鸿鹄[1] 左彦珍[2] 王爱民[3] 焦海涛[4] 

机构地区：[1]承德市中心医院肿瘤放化疗中心,河北承德067000 [2]承德医学院药理教研室,河北承德067000 [3]承德市中心医院消化科,河北承德067000 [4]承德医学院临床技能教研室,河北承德067000

出　　处：《解剖学报》2015年第6期797-801,共5页Acta Anatomica Sinica

基　　金：河北省教育厅资助项目(QN2014036);河北省卫生厅重点课题资助项目(ZL20140247);承德市科学技术研究资助项目(20142015);承德医学院自然科学基金资助项目(201407)

摘　　要：目的探索二氢杨梅素(DMY)对人肺腺癌A549细胞放射治疗敏感性的调节作用,及新的肺癌放疗增敏剂。方法采用MTT法和集落形成实验分别寻找二氢杨梅素对A549细胞的短期和长期20%抑制浓度(IC20)作为放疗增敏浓度;流式细胞术周期实验检测二氢杨梅素(IC20)作用不同时间对A549细胞周期时相的改变;集落形成实验检测二氢杨梅素(IC20)预干预24h后再照射对肺癌A549细胞放疗敏感性的影响。结果 MTT和集落形成实验均显示,DMY对A549细胞以浓度依赖形式存在短期和长期的抑制作用,MTT实验时IC20为40mg/L,集落形成实验时IC20为7mg/L;流式细胞术周期实验结果显示,二氢杨梅素作用24h时G2/M期比率增高明显(P<0.05);集落增敏实验结果显示,DMY预处理24h后对A549细胞的放射治疗并未见到显著增敏作用(P>0.05)。结论二氢杨梅素体外存在抑制A549细胞增殖的作用,其效应与药物浓度和细胞初始接种密度有关;二氢杨梅素作用24h可以使A549细胞G2/M期比率明显增加,但是单纯通过调整放疗前细胞周期时相并未见到明显的放疗增敏作用。Objective To study the radiosensitivity of dihydromyricetin （DMY） on human non-small cell lung cancer A549 ceils, and to explore a new drug for lung cancer radiotherapy sensitization. Methods MTT assay and colony formation assay were used to determined the IC20of dihydromyrieetin at different density A549 cells. Flow eytometry （FCM） was used to detect the change of A549 cell cycle phase impacted by DMY of IC20 during different time （6, 12, 24, 48hours）. Colony formation assay was used to determine the radiosensitivity on A549 cells by dihydromyricetin pre- intervention in vitro. Results The proliferation of A549 cells was inhibited in a dose-dependent manner, MTT assay showed the IC20 was 40mg/L and colony formation assay showed the IC20 was 7mg/L. FCM cycle experiment showed that the rate of Gz/M phase ratio obviously increased when A549 cells was incubated 24 hours with DMY; A significant sensibilization leading by the effect of radiotherapy sensibilization on A549 cells after DMY pre-intervention 24 hours was not detected by clone formation sensibilization assay. Conclusion The proliferation of A549 ceils was inhibited in a dose- dependent manner of dihydromyricetin, and the effect was influenced by cell density. Although the rate of Gz/M phase ratio obviously increased when A549 cells were incubated 24 hours with DMY, there was no significant sensibilization on A549 cells after DMY pre-intervention 24 hours. A further study should be made.

关 键 词：二氢杨梅素 A549细胞 放射治疗 敏感性 流式细胞术 人 

分 类 号：R734.2[医药卫生—肿瘤]