酵母源金属硫蛋白对急性铅中毒小鼠的排铅及过氧化损伤修复作用  

Effect of metallothionein from yeast on lead expelling and oxidative damage repair in mice with acute lead poinsoning

作　　者：徐炳政[1] 张东杰[1] 王颖 张桂芳 陈纯琦 王欣卉 

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]国家杂粮工程技术研究中心,黑龙江大庆163319

出　　处：《中国生物制品学杂志》2015年第11期1142-1146,共5页Chinese Journal of Biologicals

基　　金：省博士后资助项目(LBH-Z13168);黑龙江省自然基金(C201445)

摘　　要：目的探讨具有自主知识产权的两种构型酵母源金属硫蛋白(metallothionein,MT)对急性铅中毒小鼠的排铅及过氧化损伤修复作用。方法一次性经腹腔注射醋酸铅溶液(100 mg/kg),构建急性铅中毒小鼠模型,另设正常对照组(NOR组,注射生理盐水0.2 mg/kg)。将模型小鼠随机分为11组:模型对照组(Mo D组,注射生理盐水0.2 mg/kg)、阳性对照组[二巯基丁二酸(dimercaptosuccinic acid,DMSA)组,注射DMSA 0.16 mg/kg]、动物源MT(Zn-MT)低(0.16 mg/kg)、中(0.2 mg/kg)、高(0.24 mg/kg)剂量组、MT-Ⅰ低、中、高剂量组、MT-Ⅱ低、中、高剂量组,每组10只。造模2 h后,小鼠每天同一时间一次性灌胃给药0.2 ml,连续14 d。实验期间,每天观察并记录小鼠形态特征及体重变化。末次给药24 h后,每组随机选取5只小鼠,测定血清中丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(super oxide dismutase,SOD)及谷胱甘肽-过氧化物酶(GSH-Px)水平(抗氧化酶活性);各组其余5只采用石墨炉原子吸收光谱法测定血液中矿物元素铅的含量。结果小鼠急性铅染毒后,体重升高程度显著下降(P<0.05),灌胃给药14 d后,仅DMSA组小鼠中毒症状改善,体重与NOR组小鼠差异无统计学意义(P>0.05);MT处理组小鼠体重与Mo D组差异无统计学意义(P>0.05)。与MT处理组比较,DMSA组小鼠血铅含量显著下降(P<0.05),MDA含量显著升高(P<0.05)。与Mo D组比较,MT处理组小鼠血铅水平显著下降(P<0.05),且呈显著的量效关系(P<0.05),MT-Ⅰ与Zn-MT排铅效果差异无统计学意义(P>0.05),但显著高于MT-Ⅱ(P<0.05)。3种MT处理组小鼠血清中MDA、SOD及GSH-Px水平驱于正常(P<0.05),其中MT-Ⅰ与MT-Ⅱ组小鼠血清中MDA水平显著低于Zn-MT组(P<0.05),SOD与GSH-Px水平差异无统计学意义(P>0.05)。结论两种构型的酵母源MT对急性铅中毒小鼠具有显著的排铅效果,与动物源MT效果类似,并能显著提高抗氧化酶活性,减少脂质过氧化产物,从而修复铅致机�Objective To investigate the effect of two metallothioneins （MTs） from yeast, with proprietary intellectual property right, on lead expelling and oxidative damage repair in mice with acute lead poisoning. Methods Mouse model of acute lead poisoning was established by a single intraperitoneal injection with lead acetate （100 mg/ kg）, using those injected with physiological saline （0. 2 mg/kg） as control （NOR group）. The model mice were randomly divided into eleven groups, ten for each. The mice in model control （MoD） group were injected with 0. 2 mg/kg physiological saline, while those in positive control group with 0. 16 mg/kg dimereaptosuccinic acid （DMSA）. However, the mice in three Zn- MT, three MT- Ⅰ and three MT-Ⅱ groups were treated with the corresponding drugs at low （0. 16 mg/ kg）, moderate （0. 2 mg/kg） and high （0. 24 mg/kg） dosages, respectively, 2 h after establishment of model, once a day at the same time points for 14 d, in a volume of 0. 2 ml by lavage. The statuses and bodyweights of mice were observed and recorded daily. Five mice were selected randomly from each group 24 h after the last lavage and determined for malondialdehyde（MDA）, super oxide dismutase （SOD） and GSH-Px levels in sera. The other five mice in each group were determined for lead content in blood by graphite furnace atomic absorption spectrometry （GFAAS）. Results The bodyweight gains of mice decreased significantly after acute lead poisoning （P 〈 0. 05）. Only the symptoms of poisoning of mice in DMSA group were improved 14 d after lavage, of which the bodyweights showed no significant difference with those in NOR group （P 〉 0. 05）. The bodyweights of mice in MT groups showed no significant with those in MoD group （P 〉 0. 05）. Com- pared with those in MT groups, the blood lead content in DMSA group decreased significantly （P 〈 0. 05）, while the MDA content increased significantly （P 〈 0. 05）. However, compared with those in MoD group, the blood lead

关 键 词：酵母源金属硫蛋白 急性铅中毒 排铅 过氧化损伤 

分 类 号：Q939.97[生物学—微生物学] R392-33[医药卫生—免疫学]