Na^＋/Ca^2＋ Exchanger 3 is Downregulated in the Hippocampus and Cerebrocortex of Rats with Hyperthermia-induced Convulsion  

作　　者：Dan Sun Jun-Hua Xiao Yan Bai Mo-Si Chen Jia-Sheng Hu Ge-Fei Wu Bing Mao Shu-Hua Wu Yan Hu 

机构地区：[1]Department of Pediatric Neurology, Wuhan Children's Hospital, Wuhan, Hubei 430016, China [2]Department of Pharmacology, Basic Medical College, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China [3]Department of Pediatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China

出　　处：《Chinese Medical Journal》2015年第22期3083-3087,共5页中华医学杂志（英文版）

基　　金：This study wassupported by grants from the Natural Science Foundation Project of Hubei Province （No. 2004ABA234）, Wuhan Morning Program Project （No. 200850731390）, and Project of Wuhan Clinical Medical Research Center in Children＇s Neural Disease （No. 2014-160）.

摘　　要：Background： Na^＋/Ca^2＋ exchanger （NCX） plays a crucial role in pentylenetetrazol-induced convulsion. However, it is unclear whether NCX is critically involved in hyperthermia-induced convulsion. In this study, we examined the potential changes in NCX3 in the hippocampus and cerebrocortex of rats with hyperthermia-induced convulsion. Methods： Twenty-one Sprague Dawley rats were randomly assigned to control group, convulsion-prone group and convulsion-resistant group （n = 7 in each group）. Whole-cell patch-clamp method was used to record NCX currents. Both the Western blotting analysis and immunofluorescence labeling techniques were used to examine the expression of NCX3. Results： NCX currents were decreased in rats after febrile convulsion. Compared to the control group, NCX3 expression was decreased by about 40% and 50% in the hippocampus and cerebrocortex of convulsion-prone rats, respectively. Furthermore, the extent of reduction in NCX3 expression seemed to correlate with the number of seizures. Conclusions： There is a significant reduction in NCX3 expression in rats with febrile convulsions. Our findings also indicate a potential link between NCX3 expression, febrile convulsion in early childhood, and adult onset of epilepsy.Background： Na^＋/Ca^2＋ exchanger （NCX） plays a crucial role in pentylenetetrazol-induced convulsion. However, it is unclear whether NCX is critically involved in hyperthermia-induced convulsion. In this study, we examined the potential changes in NCX3 in the hippocampus and cerebrocortex of rats with hyperthermia-induced convulsion. Methods： Twenty-one Sprague Dawley rats were randomly assigned to control group, convulsion-prone group and convulsion-resistant group （n = 7 in each group）. Whole-cell patch-clamp method was used to record NCX currents. Both the Western blotting analysis and immunofluorescence labeling techniques were used to examine the expression of NCX3. Results： NCX currents were decreased in rats after febrile convulsion. Compared to the control group, NCX3 expression was decreased by about 40% and 50% in the hippocampus and cerebrocortex of convulsion-prone rats, respectively. Furthermore, the extent of reduction in NCX3 expression seemed to correlate with the number of seizures. Conclusions： There is a significant reduction in NCX3 expression in rats with febrile convulsions. Our findings also indicate a potential link between NCX3 expression, febrile convulsion in early childhood, and adult onset of epilepsy.

关 键 词：Cerebrocortex Febrile Convulsion HIPPOCAMPUS HYPERTHERMIA Na^＋/Ca^2＋ Exchanger 3 

分 类 号：Q95-331[生物学—动物学] Q426