丹参酮ⅡA对脑出血大鼠灶周Nestin、Neun表达的影响  

Effects of tanshinoneⅡA to the expression of Nestin、Neun in perihematoma with intracerebral hemorrhage models of rats

作　　者：张毅[1] 方永军[1] 周锋[1] 胡亚莉[2] 柯尊华[1] 周振国[1] 罗卫 畅涛[1] 

机构地区：[1]陕西中医药大学附属医院脑外科,咸阳712000 [2]陕西中医药大学,咸阳712000

出　　处：《陕西中医》2015年第11期1555-1558,共4页Shaanxi Journal of Traditional Chinese Medicine

基　　金：陕西省教育厅科学研究计划项目(自然科学专项)(11JK0697);陕西省科技厅科学技术研究发展计划项目(自然基金)(2012JM4004)

摘　　要：目的:观察丹参酮ⅡA对脑出血大鼠灶周Nestin、Neun阳性细胞数影响。方法:78只Sprague Dawley大鼠被随机分为4组,其中正常组6只,假手术组24只,对照组24只,治疗组24只,治疗组腹腔注射丹参酮ⅡA,立体定向仪自体血注入法制备大鼠脑出血模型,免疫组化染色观察正常组及假手术组、对照组、治疗组术后第3d、7d、14d、28d灶周Nestin、Neun的表达及神经功能评分并进行比较。结果:脑出血灶周Nestin阳性细胞数从造模后即增多,第7天时明显增多,随后持续升高,并于第14d时数量达到峰值,随后逐渐减少,第28天时仍高于正常水平;治疗组与对照组同期比较,在第7天(P<0.05)及第14天(P<0.01)差异显著。Neun阳性细胞数在造模后3d即有明显增多,至第7天时达高峰,此后逐渐下降,至第28天时仍高于正常水平;治疗组与其他组同期比较,有明显差异(P<0.01)。治疗组大鼠神经功能恢复较对照组有明显改善(P<0.05)。结论:丹参酮ⅡA可诱导脑出血大鼠灶周内源性神经干细胞增殖分化并改善神经功能。Objective：To observe the effects of TanshinoneⅡA to Nestin and Neun＇s positive cells in perihematoma with intracerebral hemorrhage models of rats.Methods：78Sprague Dawley rats were randomly divided into four groups：normal group 6,sham-operated group 24,control group 24,treatment group 24,treatment group was given an intraperitoneal injection of TanshinoneⅡA.Stereotaxic apparatus and infuse fresh quantitative autologous blood into rat＇s brain was utilized to build up the ICH model.On 3d,7d,14 d,and 28 dafter ICH,the rats were performed behavioral test,and the expression of Nestin and Neun＇s positive cells around hematoma in ICH was analyzed by immunohistochemistry.Results：The Nestin＇s positive cells started rising at the begining post operation,increased significantly at 7d,the peak appeared at 14 d,then the number gradually decline,but there were still significant different compared with other groups at28 d,compared with control group,the expression of Nestin＇s positive cells of treatment group were more obvious and early,especially at 7d（P〈0.05）and 14d（P〈0.01）.The Neun＇s positive cells started rising at the 3d,the peak appeared at7 d,then dropped gradully,at 28 d,there were still higher compared with other groups.The expression of Neun＇s positive cells of treatment group were more obvious（P〈0.01）.The rat＇s neural functional recovery of treatment group was more apparent than the control group（P〈0.05）.Conclusion：TanshinoneⅡA can activate endogenous neural stem cell proliferation and improve the neurological function.

关 键 词：脑出血/中医药疗法 丹参酮ⅡA/治疗应用 动物实验 大鼠 巢蛋白 

分 类 号：R743.34[医药卫生—神经病学与精神病学]