载淫羊藿苷缓释电纺纤维对成骨细胞影响的实验研究  

The effects of electrospun nanofibers loading icariin on osteoblast MC3T3-E1 cells

作　　者：刘童斌[1] 袁月[1] 赵钰婷[1] 王晶晶[1] 孟维艳[1] 

机构地区：[1]吉林大学口腔医院种植科,长春130021

出　　处：《实用口腔医学杂志》2015年第6期770-775,共6页Journal of Practical Stomatology

基　　金：吉林省发展与改革委员会项目(编号:2013C022-6)

摘　　要：目的:探讨含有淫羊藿苷(ICA)的左旋聚乳酸(PLLA)电纺丝纤维(ICA/PLLA)缓释效果及其对成骨细胞增殖、分化的影响。方法:通过乳液法(W/O)制备载不同浓度ICA的PLLA静电纺丝缓释纤维扫描电镜(SEM)观察其外观形貌。使用不同梯度药物测定ICA/PLLA体外释放动力学。通过荧光标记纤维观察小鼠胚胎成骨细胞前体细胞(MC3T3-E1)细胞黏附噻唑蓝法(MTT)和碱性磷酸酶(ALP)检测其增殖和分化。结果:含ICA纤维体外有效释放22 d,ICA/PLLA与细胞黏附性较好,对MC3T3-E1细胞增殖无影响(P>0.05),可显著增加成骨细胞ALP活性(P<0.05)。结论:ICA/PLLA可以有效缓释ICA并促进MC3T3-E1细胞分化。Objective：To prepare poly-L-lactic acid（PLLA） electrospun nanofibers carrying icariin（ICA）（ICA/PLLA） and to evaluate the effects of the ICA/PLLA on MC3T3-E1 cells.Methods：ICA solution was dispersed into PLLA solution,and electrospun fibers were fabricated by W/O emulsion method.The morphology of ICA/PLLA was observed by SEM.The in vitro release kinetics of ICA/PLLA was examined.The attachment of MC3T3-E1 cells on ICA/ PLLA was examined by propidiumiodide（PI） labling and observed under fluorescent microscope.The proliferation of the cells was measured by MTT assay.The differentiation of the cells was observed by alkaline phosphatase（ALP） assay.Results：In vitro,ICA was effectively released from ICA/PLLA for 22 days,cells were attached well on the surface in all groups,ICA did not affect the proliferation of MC3T3-E1 cells（P〉0.05）,but increased the ALP activity（P〈0.05） of the cells.Conclusion：ICA/PLLA can effectively control the release of ICA and promote the differentiation of MC3T3-E1 cells.

关 键 词：淫羊藿苷(ICA) 控制释放 成骨细胞 左旋聚乳酸(PLLA) 

分 类 号：R783.1[医药卫生—口腔医学]