人肌球蛋白轻链9在前列腺癌的表达水平及其对生化复发的预测价值  

Expression level and predictive value of myosin light chain 9 in biochemical recurrence of prostate cancer

作　　者：张增强[1] 叶永康[1] 黄亚强[2] 吴永定 李牧[1] 何慧婵[2] 

机构地区：[1]东莞市人民医院泌尿外科,东莞523059 [2]广州医科大学广州市第一人民医院泌尿外科,广州510180

出　　处：《新医学》2015年第11期732-736,共5页Journal of New Medicine

基　　金：广东省科技计划项目(2013B021800055)

摘　　要：目的研究人肌球蛋白轻链9(MYL9)在前列腺癌中的表达水平及其对临床生化复发的预测价值。方法利用蛋白质组学技术检测前列腺癌及癌旁良性前列腺组织MYL9的表达水平,再通过蛋白免疫印迹法验证蛋白质组学结果,最后使用Taylor的数据对MYL9进行生物信息学分析,分析不同年龄、血清PSA水平、Gleason评分、病理分期、转移与否及生存与否的前列腺癌患者间MYL9水平的差异。结果蛋白质组学结果显示,MYL9在前列腺癌组织中的表达水平比癌旁良性前列腺组织下调5.67倍(P<0.01);蛋白免疫印迹法结果也显示,前列腺癌组织中MYL9表达水平比癌旁良性前列腺组织明显下调(0.23±0.08 vs.0.73±0.06,P<0.001);前列腺癌患者中,年龄<66岁、Gleason评分<8分、病理分期<T3A、肿瘤无转移、存活者的MYL9表达水平明显高于年龄≥66岁、Gleason评分≥8分、病理分期≥T3A、肿瘤发生转移、死亡者(P均<0.001),血清PSA水平是否<4μg/L对MYL9表达无影响(P>0.05)。MYL9低表达组前列腺癌患者的无生化复发生存率明显低于MLY9高表达组(χ2=11.575,P<0.001)。结论 MYL9在前列腺癌中比癌旁前列腺组织表达下调,且MYL9表达水平较低者的临床预后较差。Objective To investigate the expression level and predictive value of myosin light chain 9 （MYL9）in the biochemical recurrence of prostate cancer.Methods The expression levels of MYL9 in the prostate cancer and adjacent benign prostate tissues were assessed by proteomics technique and the results were further validated by western blot.Eventually,the bioinformatics analysis of MYL9 was performed using Taylor data to analyze the difference in the expression of MYL9 among prostate cancer patients with different ages,ser-um PSA levels,Gleason scores,pathological stages,whether metastasis or not and whether survived or not. Results Proteomics results revealed that the expression level of MYL9 in the prostate cancer tissue was down-regulated by 5.67 times compared with that in the adjacent benign prostate tissue （P 〈0.01）;Western blot a-nalysis indicated that the expression level of MYL9 in the prostate cancer tissue was 0.23 ±0.08,significantly lower compared with 0.73 ±0.06 in the adjacent benign prostate tissue （P 〈0.001）;The expression of MYL9 in prostate cancer patients aged 〈66 years,with Gleason score 〈8,pathological staging 〈T3A,no tumor me-tastasis and the survived was significantly higher than that in their counterparts aged ≥66 years,Gleason score≥8,with pathological staging ≥T3A,tumor metastasis and the dead （all P 〈0.001）.Whether serum PSA level 〈4 μg/L exerted no effect on the expression of MYL9 （P 〉0.05）.The biochemical recurrence-free sur-vival rate in prostate cancer patients with low expression of MYL9 was significantly lower compared with that in their counterparts highly expressing MLY9 （χ^2 =11.575,P 〈0.001）.Conclusions MYL9 expression in the＆amp;nbsp;prostate cancer tissue was down-regulated compared with that in adjacent normal prostate tissue.Patients with lower expression level of MYL9 yielded worse clinical prognosis.

关 键 词：前列腺癌 肌球蛋白轻链 蛋白质组学 临床预后 

分 类 号：R737.25[医药卫生—肿瘤]