硫酸钠对蛋鸡生产性能、抗氧化能力、肠道形态及盲肠微生物菌群的影响  

Effects of Sodium Sulfate on Performance,Antioxidant Capacity,Intestinal Morphology and Cecal Microflora Population of Laying Hens

作　　者：熊平文 刘兵[1] 陈娜娜[1] 何俊娜[1] 余东游[1] 

机构地区：[1]浙江大学动物科学学院,农业部华东动物营养与饲料重点实验室,杭州310058

出　　处：《动物营养学报》2015年第11期3358-3365,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：东南沿海地区大学农业科技服务技术集成与示范(2013BAD20B02)

摘　　要：本试验旨在研究饲粮中添加硫酸钠对蛋鸡生产性能、抗氧化能力、肠道形态及盲肠微生物菌群的影响。选取健康且体重和产蛋率相近的20周龄海兰白蛋鸡450只,随机分为5组,每组6个重复,每个重复15只鸡。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加0.3%、0.6%、1.5%、3.0%硫酸钠的试验饲粮。试验期8周。结果显示：1）饲粮中添加硫酸钠可促进蛋鸡产蛋,其中以低剂量（0.3%和0.6%）组效果较佳,但差异不显著（P〉0.05）。2）与对照组相比,饲粮中添加硫酸钠对血浆和肝脏的抗氧化指标均无显著影响（P〉0.05）。与0.6%组相比,高剂量（1.5%和3.0%）组血浆的谷胱甘肽过氧化物酶（GSH-Px）活性和肝脏的总超氧化物歧化酶（T-SOD）活性显著或极显著降低（P〈0.05或P〈0.01）,3.0%组的血浆总抗氧化能力（T-AOC）和肝脏GSH-Px活性显著降低（P〈0.05）。3）与对照组相比,0.6%组十二指肠和回肠的绒毛高度分别提高了30.51%和42.63%（P〈0.01）,0.6%组十二指肠的隐窝深度显著降低（P〈0.05）,绒隐比显著提高（P〈0.05）。与0.6%组相比,高剂量（1.5%和3.0%）组十二指肠和回肠的绒毛高度及十二指肠的绒隐比极显著降低（P〈0.01）,3.0%组十二指肠和回肠的隐窝深度极显著增加（P〈0.01）。4）与对照组相比,低剂量（0.3%和0.6%）组极显著降低了大肠杆菌数量（P〈0.01）,且以0.6%组效果最佳。与0.6%组相比,高剂量（1.5%和3.0%）组肠杆菌和大肠杆菌数量显著或极显著增加（P〈0.05或P〈0.01）。本试验结果提示,饲粮中添加0.3%-0.6%的硫酸钠能提高蛋鸡的抗氧化能力,改善肠道形态和调节微生物菌群,且蛋鸡饲粮中硫酸钠适宜添加水平为0.6%。This experiment was conducted to evaluate the effects of sodium sulfate supplementation in laying hens diet by determining performance,antioxidant capacity,intestinal morphology and cecal microflora population. Four hundred and fifty 20-week-old Hyline W hite laying hens with similar body weight and laying rate were randomly allocated to 5 groups with 6 replicates per group and 15 hens per replicate. Hens in the control group was fed a basal diet,and hens in experimental groups were fed the basal diets supplemented with 0.3%,0.6%,1.5%,3.0% sodium sulfate,respectively. The trial lasted for 8 weeks. The results showed as follows：1） diets supplemented with sodium sulfate tended to improve the laying performance of laying hens with no significant effect（ P 〉0.05）,and the effect of lowdose groups（ 0.3% and 0.6%） was better. 2） Compared with control group,diets supplemented with sodium sulfate had no significant influences on antioxidant parameters（ P 〉0.05）. Compared with 0.6% group,the activities of glutathione peroxidase（ GSH-Px） in plasma and total superoxide dismutase（ T-SOD） in liver of high dose（ 1. 5% and 3. 0%） groups were significantly decreased（ P 〈0.05 or P 〈0.01）,group added by 3.0% had significantly reduced the total antioxidant capacity（ T-AOC）in plasma and the activity of GSH-Px in liver（ P 〈0.05）. 3） Compared with control group,the villus height in duodenum and ileum of 0.6% group had significantly raised by 30. 51% and 42. 63%（ P 〈0. 01）; the crypt depth in duodenum was significantly reduced（ P 〉0.05） and the ratio of villus height to crypt depth（ V / C） in duodenum was significantly raised（ P 〉0.05）. Compared with 0.6% group,the villus height in duodenum and ileum and V / C in duodenum of high dose（ 1.5% and 3.0%） groups were significantly decreased（ P 〈0.01）,and the crypt depth in duodenum and ileum of 3. 0% group was significantly increased（ P 〈0. 01）. 4） Compared with control group,0. 3% and 0. 6% groups

关 键 词：硫酸钠 蛋鸡 生产性能 抗氧化能力 肠道形态 微生物菌群 

分 类 号：S816.71[农业科学—饲料科学] S831.1[农业科学—畜牧学]