DA-6对冬季迟缓期的艾纳香生长和有效成分含量的影响  

Effect of DA-6 on Growth and Contents of Effective Constituents in Blumea Balsamifera in Slow Growth Period of Winter

作　　者：王丹[1] 马青松[1] 范佐旺[1] 李小婷[1] 宛骏 袁蕾[1] 陈晓鹭 邹纯礼 庞玉新[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点开放实验室/海南省艾纳香工程技术研究中心,海南儋州571737 [2]海南艾纳香科技发展有限公司,海南海口570208

出　　处：《中国现代中药》2015年第11期1184-1187,1192,共5页Modern Chinese Medicine

基　　金：国家自然科学基金项目(81403035);科技型中小企业技术创新基金(14C26214602908)

摘　　要：目的:研究DA-6对冬季迟缓期艾纳香生长和有效成分含量的影响。方法:以一年生艾纳香种子苗为实验材料,在冬季艾纳香生长迟缓期进行3次喷施DA-6。测定艾纳香的株高、地径、叶长和叶宽等生长指标以及叶干重。分别采用紫外可见分光光度法和GC法测定艾纳香叶片总黄酮和l-龙脑的相对含量,并计算绝对含量。结果:DA-6显著促进艾纳香生长,增加叶干重,其中10 mg·L-1DA-6作用的效果最显著。DA-6抑制了艾纳香叶片中总黄酮和l-龙脑相对含量的积累。CK处理组总黄酮和l-龙脑相对含量最高,显著高于100 mg·L-1DA-6处理组77.78%和96.49%。DA-6显著提高了总黄酮和l-龙脑的绝对含量。10 mg·L-1DA-6处理组艾纳香叶片中总黄酮和l-龙脑含量最高,显著高于其他处理组,是CK处理组的2.33倍和3.33倍。结论:因此,在冬季艾纳香生长迟缓期施加DA-6可以促进艾纳香生长和叶干重的增加,提高总黄酮和l-龙脑的绝对含量。Objective： To investigate the effect of DA-6 on growth and contents of effective constituents in Blumea balsamifera （L.） DC. in slow growth period of winter. Methods： The seedlings of one-year-old B. balsamifera were applied with DA-6 in slow growth period of winter three times. The height, ground diameter, length of leaf, width of leaf and leaf biomasses were measured. Then, the relative contents of total flavonoids and /-borneol in leaves of B. balsamifera were determined by UV and GC methods, respectively. The absolute contents were calculated. Results： The DA-6 significantly enhanced the growth of B. balsamifera, and increased leaf biomass. The effect of 10 mg. L-1 DA-6 treatment group was the most obvious. The application of DA-6 significantly inhibited the accumulation of relative contents of total flavonoids and /-bomeol. The relative contents of total flavonoids and l-borneol under CK treatment were the highest, which were over 100 mg. L-1 DA- 6 treatment with 77.78% and 96. 49%. However, DA-6 significantly increased the absolute contents of total flavonoids and /-borneol in leaves. The absolute contents of total flavonoids and/-borneol under 10 mg-L- 1 DA-6 treatment were the highest, which were 2. 33 and 3.33 times than that of CK. Conclusion ： Therefore, the DA-6 could significantly promote the growth and the accumulation of leaves, as well as the absolute contents of total flavonoids and l-borneol in B. balsamifera in slow growth period of winter.

关 键 词：DA-6 艾纳香 迟缓期 l-龙脑 总黄酮 叶 

分 类 号：R153.2[医药卫生—营养与食品卫生学]