液基细胞学和HPV原位杂交检测在宫颈病变筛查中的应用  

Application of liquid-based cytology and HPV in situ hybridization detection in cervical lesions screening

作　　者：张峰[1] 王洪远[1] 李建新[1] 刘名霞 

机构地区：[1]山东省日照市中医医院检验科,山东日照276800

出　　处：《医学检验与临床》2015年第5期11-13,共3页Medical Laboratory Science and Clinics

摘　　要：目的：探讨薄层液基细胞学（TCT）和HPV原位杂交检测在宫颈病变筛查中的应用。方法：对日照市中医医院妇科门诊1257例接受宫颈病变筛查的患者进行TCT检查和HPV28种基因分型检测，并对TCT异常、HPV阳性患者行阴道镜下宫颈组织病理学检查。结果：以组织病理学为判定标准，CINⅠ、CIN II、CIN III及宫颈癌组的 HPV感染率和TCT阳性率显著高于正常和炎症组，差异有统计学意义（P〈0.05），并随着宫颈病理学分级的增高， HPV感染率也相应增高。联合应用液基细胞学和HPV原位杂交检测灵敏度（97.7%）、阴性预测值（98.8%）均有不同程度的提高，与单纯行TCT检测或HPV DNA检测的敏感度、阴性预测值相比，差异有统计学意义（P〈0.05）。结论：TCT和HPV原位杂交检测联合应用能够提高宫颈癌及癌前病变的检出率，是宫颈癌筛查有效的方法。Objective：To investigate the value and clinical significance of the TCT and HPV DNA test in cervical lesions screening.Methods：Cervcal exfoliate cell from 1257 women were collected to detect TCT and 28 HPV DNA test. Women with abnormai results of TCT and HPV DNA were examined by colposcopy and biopsy.Results： HPV infection rate and TCT postive rate of CIN I group,CIN II group ,CIN III group and cervical cancer group was higher than those in normal and inflammatory（P〈0.05） . The grades of the pathology are related with the HPV postive rate in the precancerous lesion of cervical cancer. HPV infection rate also was increased by the rise of pathological level accordingly.The sensitivity,specificity,negative predictive value of combined examination of both tests improved to varying degrees and they were 97.7% and and 98.8%.respectively,with significant difference between them（P〈0.05）.Conclusions：TCT and HPV DNA test will increase the detected rate of the diagnosis of precancerous lesion of cervical cancer..It can be an effective method in cervical cancer screening.

关 键 词：子宫颈肿瘤 人乳头状瘤病毒 液基薄层细胞学 筛查 

分 类 号：R737.33[医药卫生—肿瘤]