高效液相色谱法测定化妆品中的乐杀螨和克螨特含量  

Determination of Binapacryl and Propargite in Cosmetics by HPLC

作　　者：顾宇翔[1] 熊薇[1] 戴彦韵[1] 

机构地区：[1]上海市质量监督检验技术研究院,上海200233

出　　处：《香料香精化妆品》2015年第5期54-58,共5页Flavour Fragrance Cosmetics

基　　金：上海市科委技术标准专项(编号14DZ0501102)

摘　　要：建立了以高效液相色谱法检测化妆品中乐杀螨和克螨特的方法。样品用乙腈提取，超声提取15min，以8000r／min的转速离心3min，取上清液加入Florosil粉进行纯化，再以8000r／min转速离心3min，取上清液分析。采用SBC18柱（250mm×4．6mm×5μm），流动相为水-乙腈（体积比26：74-）洗脱，检测波长为224nm。乐杀螨检出限和定量限分别为7和20mg／kg，克螨特检出限和定量限分别为16和50mg／kg（以3、10倍信噪比计算得出），加标回收率分别为90．2％～106．4％（乐杀螨）和80．4％～103．2％（克螨特）。该方法简便、准确，适用于对化妆品中乐杀螨和克螨特的检测。A HPLC method to determine binapacryl and propargite in cosmetics was developed. Samples were ultrasonically extracted for 15 min with acetonitrile, then centrifugated at 8 000 r/min for 3 min. Florosil powder was added to obtained supematant, then it was centrifugated at 8 000r/min for 3 min again. Finally the supernatant was analyzed by SB Cl8 column （251） mm × 4. 6 mm × 5 μm）. The mobile phase was water-acetonitrile （26/74, volume ratio）, detection wavelength was 224 nm. The limit of detection and quantitation of binapacryl were 7 and 20 mg/kg, that of propargite were 16 and 51） mg/kg （S/N = 3 and 10）, respectively. The recoveries of the method were in the range of 90. 2% to 106. 4% （binapacryl） and 80. 4% to 103.2% （propargite）. The established method was simple and accurate, it was suitable for analysis of binapacryl and propargite in cosmetics.

关 键 词：化妆品 乐杀螨 克螨特 高效液相色谱法 测定 

分 类 号：O657.72[理学—分析化学]