Cu^(2+)、Zn^(2+)和Al^(3+)对异补骨脂素与牛血清白蛋白相互作用的影响  

Effects of Cu^(2+)、Zn^(2+) and Al^(3+) on the Interaction Between ISO and BSA

作　　者：彭婷婷[1] 

机构地区：[1]白城师范学院化学学院,吉林白城137000

出　　处：《白城师范学院学报》2015年第11期32-36,共5页Journal of Baicheng Normal University

摘　　要：本文采用荧光光谱法探究在18℃、40℃下,Cu^(2+)、Zn^(2+)和Al^(3+)金属离子对异补骨脂素(ISO)与牛血清蛋白(BSA)相互作用的影响,并分别测定了两个温度下的猝灭常数Ksv、结合常数K_A和结合位点数n.结果表明,三种离子的存在使得异补骨脂素对BSA的内源荧光猝灭效应有所增强,并且改变了二者的作用力类型.This paper explores the effects of such metal ions as Cu^(2+)、Zn^(2+) and Al^(3+) on the interaction between isopsoralen( ISO) and bovine serum albumin( BSA) by fluorescence spectrometry at 18 ℃ and 40 ℃.Moreover,the quenching constant Ksv,binding constant KA and the numbers of the binding sites at the two different temperatures were calculated. The results suggest that the three metal ions enhance the quenching effect of ISO on the intrinsic fluorescence of BSA,and the types of interaction forces were changed.

关 键 词：荧光光谱 异补骨脂素 牛血清白蛋白 相互作用 

分 类 号：R969.2[医药卫生—药理学]