LBH589对OVCAR-3细胞增殖、侵袭和血管内皮生长因子表达的影响  

Effects of LBH589 on proliferation,invasion,and VEGF expression of OVCAR-3 cells

作　　者：晁宏图[1] 李晓凤[1] 寇馨歆 李雷[1] 杨晓霞[1] 王莉英[1] 王莉[1] 

机构地区：[1]河南省肿瘤医院(郑州大学附属肿瘤医院)妇瘤科,郑州450003

出　　处：《郑州大学学报（医学版）》2015年第6期769-773,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技厅重点科技攻关项目142102310434

摘　　要：目的:观察LBH589对上皮性卵巢癌OVCAR-3细胞增殖、侵袭和VEGF表达的影响。方法:采用MTT法和Transwell实验分别检测1、5、10μmol/L的LBH589对OVCAR-3细胞活性和侵袭细胞数的影响,采用Western blot法检测Ki67、MMP-2、VEGF、p-PI3K、p-Akt蛋白的表达水平。采用MTT和Western blot法分别检测PI3K抑制剂LY294002对细胞活性以及MMP-2、VEGF表达水平的影响。结果:LBH589降低OVCAR-3细胞活性(F=5.324,P<0.001),减少穿过Transwell基质膜的细胞数量(F=84.325,P<0.001),Ki67、MMP-2、VEGF、p-PI3K、p-Akt蛋白的表达水平亦降低(F=9.191、7.623、6.340、6.221、4.690,P均<0.001)。与对照组相比,LY294002抑制MMP-2和VEGF蛋白的表达,并降低OVCAR-3细胞活性(P均<0.05)。结论:LBH589可能通过调控PI3K/Akt信号通路下调MMP-2和VEGF表达水平,继而抑制上皮性卵巢癌OVCAR-3细胞增殖、侵袭和血管生成。Aim：To observe the effects of LBH589 on the proliferation, invasion and VEGF expression of OVCAR-3 cells.Methods：The effects of 1, 5, and 10μmol/L LBH589 on the relative viability and invasion of OVCAR-3 cells were determined using MTT and Transwell assay , respectively.The effects of LBH589 on the expression levels of Ki67, MMP-2, VEGF, p-PI3K, and p-Akt were measured using Western blot analysis .Finally, the effects of PI3K inhibitor LY294002 on the relative viability and the expression levels of MMP-2 and VEGF were detected using MTT assay and Western blot analy-sis, respectivelyR. esutl s：LBH589 lowered the relative viability of OVCAR-3 cells（F=5.324,P〈0.001）, reduced the number of transferred cells（F=84.325,P〈0.001）.Also, the expression levels of Ki67, MMP-2, VEGF, p-PI3K, and p-Akt decreased（F =9.191,7.623,6.340,6.221,4.690,P〈0.001）.Compared with the control group, LY294002 down-regulated the expressions of MMP-2 and VEGF, and lowered the relative viability of OVCAR-3 cells（P〈0.05）. Conclusion：LBH589 down-regulates the expressions of MMP-2 and VEGF by mediating the PI3K/Akt signaling pathway, resulting in the inhibition of proliferation , invasion and angiogenesis of OVCAR-3 cells.

关 键 词：组蛋白去乙酰化酶抑制剂 OVCAR-3细胞 增殖 侵袭 VEGF PI3K/AKT通路 

分 类 号：R737.31[医药卫生—肿瘤]